循环游离DNA的定量检测及面临的问题

Quantification of circulating cell free DNA and the existing problems

循环游离DNA(circulating cell free DNA,cfDNA)与人类疾病之间存在着密切联系。近年来,cfDNA做为一种无创检测生物标志物,展现出临床应用前景。人们针对其含量较低的特点,进行了大量研究,开发出了各种较为可靠的定量检测方法。但目前cfDNA的定量检测仍然面临问题及争议,缺乏规范和标准化的流程。本文对cfDNA的来源组成、定量检测及存在的问题进行了归纳整理及分析,希望能对提高cfDNA的定量检测的可靠性提供有益的参考。

Circulating cell free DNA(cfDNA) are closely related to human disease. In recent years,as a noninvasive biomarkers, cfDNA showed great prospect for clinical application. Because of its extremely low content, a large number of studies have been done to develop various methods for quantitative detection. At the moment cfDNA quantitative detection still faces problems such as the lack of specification and standardization in detection processes. In this paper, we reviewed the source of cfDNA, quantitative methods and existing problems of detection, and hope to give a beneficial reference for improving the reliability of the quantitative detection.