超声促释载紫杉醇纳米复合凝胶系统抑制大鼠C6胶质瘤细胞生长的实验研究

Paclitaxel-loaded nanoparticle gel system combined with ultrasonic irradiation inhibits growth of rat C6 glioma cells

目的:探究载紫杉醇纳米复合凝胶系统及其结合超声辐照对C6胶质瘤细胞生长的抑制作用。方法:分为对照组、空白纳米复合凝胶组、游离紫杉醇组、载紫杉醇纳米复合凝胶组、超声辐照载紫杉醇纳米复合凝胶组,分别处理C6胶质瘤细胞,利用四唑盐(methyl thiazolyl tetrazolium,MTT)比色法检测C6细胞的相对生长率(relative growth rate,RGR),观察各组细胞生长情况。结果:对照组与空白纳米复合凝胶组比较RGR差异无统计学意义,含药物的游离紫杉醇组、载紫杉醇纳米复合凝胶组、超声辐照载紫杉醇纳米复合凝胶组均有明显的细胞增殖抑制作用(P<0.05)。与游离紫杉醇组比较,载紫杉醇纳米复合凝胶组RGR减低,差异有统计学意义(P<0.05),载紫杉醇纳米复合凝胶组与超声辐照载紫杉醇纳米复合凝胶组之间RGR差异有统计学意义(P<0.05)。结论:载紫杉醇纳米复合凝胶系统的制备材料具有良好的生物相容性,紫杉醇可明显抑制C6胶质瘤细胞生长。载紫杉醇纳米复合凝胶影响紫杉醇对细胞的生长抑制作用,具有缓释作用,超声辐照可促进载紫杉醇纳米复合凝胶内药物释放。

Objective: To observe the inhibitory effects of paclitaxel(PTX)-loaded nanoparticle(NP) gel system combined with ultrasonic irradiation on the growth of C6 glioma cells. Methods: Five groups were set up: Control, BLANK-NP-GEL, PTX, PTX-NP-GEL and PTX-NP-GEL-US. They were administered to C6 glioma cells respectively. The relative growth rate(RGR) of cells was detected by methyl thiazolyl tetrazolium(MTT) method, and the inhibitory effects on the growth of C6 glioma cells were observed. Results: The nano materials had no obvious inhibitory effects on cell proliferation. Paclitaxel could obviously inhibit cell proliferation compared to control group(P<0.05). There were significant differences in RGR among PTX, PTX-NP-GEL and PTXNP-GEL-US groups(P<0.05). Conclusion: The selected nanoparticle gel system materials have fine biocompatibility. Paclitaxel can inhibit the growth of C6 glioma cells. The paclitaxel-loaded nanoparticle gel system has sustained release effect, and ultrasonic irradiation can promote drug release.