期刊文献+

β微管蛋白Ⅲ型及微管相关蛋白2在胶质瘤细胞中的表达 被引量:1

Expression ofclass Ⅲ β-tubulin and MAP-2 in glioma cells
下载PDF
导出
摘要 目的:研究β微管蛋白Ⅲ型(classⅢβ-tubulin)及微管相关蛋白2(MAP-2)在胶质瘤细胞中的表达。方法:购买A172,U87,U251,LN229胶质瘤细胞,进行细胞培养,real-time PCR。结果:在含胎牛血清的完全DMEM培养基中培养结果表明,在神经元干细胞培养基中进行3天和3周的培养后,A172,U87,U251,LN229中MAP-2均具有显著较低的表达量(P<0.05);在神经元干细胞培养基中进行3天的培养后,A172,U87,U251,LN229中classⅢβ-tubulin具有显著较低的表达量(P<0.05);在神经元干细胞培养基中进行3周的培养后,A172,U87,U251中classⅢβ-tubulin具有显著较低的表达量(P<0.05),但是LN229中具有显著较高的表达量(P<0.05)。在不含胎牛血清的完全DMEM培养基中培养结果表明,在神经干细胞培养基中进行4天的培养后,A172、LN229、U251中MAP-2均具有显著较高的表达量(P<0.05);U87中MAP-2具有显著较低的表达量(P<0.05)。在神经干细胞培养基中进行3周的培养后,A172、LN229、U251、U87中MAP-2均具有显著较高的表达量(P<0.05)。培养后4d和3周后A172、LN229、U251、U87中classⅢβ-tubulin表达量之间的差异不显著(P>0.05)。结论:β微管蛋白Ⅲ型及微管相关蛋白2在胶质瘤细胞中的表达均随着周围环境的变化而变化。 Objective To study the expression of class Ⅲ β-tubulin and MAP-2 in glioma cells. Methods A172, U87, U251, LN229 were purchased for cell culture, real-time PCR. Results In neural stem cell culture medium were cultured for 3 days and 3 weeks after A172, U87, U251, LN229 in MAP-2 had a significantly lower expression level(P<0.05); neural stem cells in the culture medium after 3 days, A172, U87, U251, LN229 in class Ⅲ β-tubulin has the expression was significantly lower(P<0.05); neural stem cells in the culture medium after 3 weeks, A172, U87, U251 in class Ⅲ β-tubulin expression was significantly lower with the(P<0.05), but had significantly higher expression in LN229(P<0.05). The results show that the medium completely DMEM containing fetal bovine serum, neural stem cells in the culture medium after 4 days, expression of A172, LN229 and U251 in MAP-2 were significantly higher(P<0.05); U87 MAP-2 has expressed significantly lower(P<0.05). After 3 weeks of culture in the neural stem cell medium, A172, LN229, U251 and U87 in MAP-2 had significantly higher expression(P<0.05). There was no significant difference in the expression of class Ⅲ β-tubulin between A172, LN229, U251 and U87 after 4D and 3 weeks of culture(P>0.05).Conclusion The expression of class Ⅲ β-tubulin and microtubule associated protein 2 in glioma cells changes with the surrounding environment.
出处 《影像研究与医学应用》 2018年第6期11-13,共3页 Journal of Imaging Research and Medical Applications
关键词 β微管蛋白Ⅲ型 及微管相关蛋白2 胶质瘤细胞 表达 class Ⅲ β-tubulin MAP-2 Glioma cells Expression
  • 相关文献

参考文献4

二级参考文献37

  • 1Guduler E,Guner OS,Tumay LV,et al.Serum annexin A2 levels in patients with colon cancer in comparison to healthy controls and in relation to tumor pathology[J].Med Sci Monit,2014,20:1801-1807.
  • 2Cai J.Roles of transcriptional factor Snail and adhesion factor E-cadherin in clear cell renal cell carcinoma[J].Exp Ther Med,2013,6(6):1489-1493.
  • 3Vincent A, Bien CG, Irani SR, et al. Autoantibodies associated with diseases of the CNS: new developments and future challenges[J]. Lancet Neurol, 2011, 10 (8) : 759-772.
  • 4Irani SR, Alexander S , Waters P, et al. Antibodies to K vl potassium channel-complex proteins leucine-rich, glioma inactivated 1 protein and contactin-associated protein-2 in limbic encephalitis, Morvan' s syndrome and acquired neuromyotonia[J]. Brain, 2010, 141 (9): 2734-2748.
  • 5Lai M, Huijbers MG, Lancaster E, et al. Investigation of LGIl as the antigen in limbic encephalitis previously attributed to potassium channels: a case series[J]. Lancet Neurol, 2010, 9 (8): 776-785.
  • 6Merchut MP. Management of voltage-gated potassium channel antibody disorders[J]. Neurol Clin, 2010, 28(4): 941-959.
  • 7Wong SH, Saunders MD, Lamer AJ, et al. An effective immunotherapy regimen for VGKC antibody-positive limbic encephalitis[J].J Neurol Neurosurg Psychiatry, 2010, 81 (10) : 1167-1169.
  • 8Shin YW, Lee ST, ShinJW, et al. VGKC-complexiLGIl?antibody encephalitis: Clinical manifestations and response to immunotherapy[J].J Neuroimmunology, 2013, 265(1-2): 75- 8l.
  • 9Irani SR, Michell A W, Lang B, et al. Faciobrachial dystonic seizures precede Lgil antibody limbic encephalitis[J]. Ann Neurol, 2011 , 69 (5) : 892-900.
  • 10Andrade DM, Tai P, DalmauJ, et al. Tonic seizures: a diagnostic clue of anti-LGIl encephalitis ?[J]. Neurology, 2011,76(15): 1355-1357.

共引文献21

同被引文献6

引证文献1

二级引证文献3

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部