摘要
目的:研究β微管蛋白Ⅲ型(classⅢβ-tubulin)及微管相关蛋白2(MAP-2)在胶质瘤细胞中的表达。方法:购买A172,U87,U251,LN229胶质瘤细胞,进行细胞培养,real-time PCR。结果:在含胎牛血清的完全DMEM培养基中培养结果表明,在神经元干细胞培养基中进行3天和3周的培养后,A172,U87,U251,LN229中MAP-2均具有显著较低的表达量(P<0.05);在神经元干细胞培养基中进行3天的培养后,A172,U87,U251,LN229中classⅢβ-tubulin具有显著较低的表达量(P<0.05);在神经元干细胞培养基中进行3周的培养后,A172,U87,U251中classⅢβ-tubulin具有显著较低的表达量(P<0.05),但是LN229中具有显著较高的表达量(P<0.05)。在不含胎牛血清的完全DMEM培养基中培养结果表明,在神经干细胞培养基中进行4天的培养后,A172、LN229、U251中MAP-2均具有显著较高的表达量(P<0.05);U87中MAP-2具有显著较低的表达量(P<0.05)。在神经干细胞培养基中进行3周的培养后,A172、LN229、U251、U87中MAP-2均具有显著较高的表达量(P<0.05)。培养后4d和3周后A172、LN229、U251、U87中classⅢβ-tubulin表达量之间的差异不显著(P>0.05)。结论:β微管蛋白Ⅲ型及微管相关蛋白2在胶质瘤细胞中的表达均随着周围环境的变化而变化。
Objective To study the expression of class Ⅲ β-tubulin and MAP-2 in glioma cells. Methods A172, U87, U251, LN229 were purchased for cell culture, real-time PCR. Results In neural stem cell culture medium were cultured for 3 days and 3 weeks after A172, U87, U251, LN229 in MAP-2 had a significantly lower expression level(P<0.05); neural stem cells in the culture medium after 3 days, A172, U87, U251, LN229 in class Ⅲ β-tubulin has the expression was significantly lower(P<0.05); neural stem cells in the culture medium after 3 weeks, A172, U87, U251 in class Ⅲ β-tubulin expression was significantly lower with the(P<0.05), but had significantly higher expression in LN229(P<0.05). The results show that the medium completely DMEM containing fetal bovine serum, neural stem cells in the culture medium after 4 days, expression of A172, LN229 and U251 in MAP-2 were significantly higher(P<0.05); U87 MAP-2 has expressed significantly lower(P<0.05). After 3 weeks of culture in the neural stem cell medium, A172, LN229, U251 and U87 in MAP-2 had significantly higher expression(P<0.05). There was no significant difference in the expression of class Ⅲ β-tubulin between A172, LN229, U251 and U87 after 4D and 3 weeks of culture(P>0.05).Conclusion The expression of class Ⅲ β-tubulin and microtubule associated protein 2 in glioma cells changes with the surrounding environment.
出处
《影像研究与医学应用》
2018年第6期11-13,共3页
Journal of Imaging Research and Medical Applications