替硝唑口腔贴膜对实验性大鼠牙周炎的治疗作用及其对口腔粘膜刺激性研究

Therapeutic effects of tinidazole membrane on experimental periodontitis and its oral mucosa irritation in rats

目的 :观察替硝唑口腔贴膜 (TM)对实验性大鼠牙周炎的治疗作用及其对大鼠口腔粘膜的刺激性。方法 :用丝线缝扎大鼠上颌右侧第一磨牙、肌注醋酸强的松龙以及饲喂高糖水软饲料的方法造成实验性大鼠牙周炎模型 ,将TM贴敷于病变牙周部位 ,观察其对实验性牙周炎的治疗作用 (TM 0 .0 75、0 .175、0 .375mg 只 ,每日 1次 ,连续 7d) ,观察TM对正常大鼠口腔粘膜的刺激性。每次给予TM1.5mg·cm-2 (0 .5cm× 0 .5cm ) ,每日 1次 ,连续7d。结果 :TM 0 .0 75、0 .175mg 只对大鼠的口腔牙周软组织炎症有改善作用 ,其疗效与阳性组 (灌胃给药替硝唑 10 0mg·kg-1)相似 ,TM 0 .375mg 只可使牙周软组织炎症明显减轻 ,其疗效优于阳性组。对于牙周袋的形成及牙槽骨的破坏 ,TM均无明显改善作用。对大鼠口腔粘膜无明显刺激作用。结论 :替硝唑口腔贴膜对实验性大鼠牙周炎有较好的治疗作用 。

AIM: To investigate the therapeutic effects of tinidazole membrane(TM) on experimental periodontitis and its irritation on oral mucosa in rats. METHODS: SD rats were ligated with silk floss on the first molar of right upper jaw,injected (im) with hydrocortidelt and fed with special food to induce experimental periodontitis. Model animals were treated with TM by pasted on the lesion region for 7 days in succession(once a day), then the therapeutic effects were observed. The irritation of TM on the oral mucosa in rats was observed after TM were administrated 7 days. RESULTS: TM 0.075 , 0.175 and 0.375 mg per rat had significantly therapeutic effect on the soft tissues inflammation of experimental periodontitis, and their effects were as same as or superior to tinidazole 100 mg·kg -1 (ig). After TM 1.5 mg·cm -2 ( 0.5 cm × 0.5 cm ) were treated for 7 days in succession through pasted on the inside cheek,and the oral mucosa had no significant change. CONCLUSION: TM has significantly therapeutic effect on experimental periodontitis and no significant oral mucosa irritation in rats.