大鼠脑出血后一氧化氮合酶阳性神经元数目的时程变化及果糖二磷酸钠镁干预作用

Timed number of nitric oxide synthase-positive cells in brain of rats with intracerebral hemorrhage and influence of sodium magnesium fructose diphosphate

目的 :研究脑出血后一氧化氮合酶 (NOS)阳性神经元的时程变化 ,以及果糖二磷酸钠镁 (FDPM )的干预作用。方法 :以胶原酶诱导法制备大鼠脑出血模型 ,应用NADPH黄递酶组织化学方法 ,观察脑出血后NOS阳性神经元随时间的变化过程 ,并观察FDPM对其影响。结果 :脑出血后NOS阳性神经元2h时明显增多 ,6h有轻度下降 ,12h再度增多 ,2 4～ 72h达高峰 ,7d (16 8h)下降 ,但仍明显高于正常。脑出血后使用 4 0 0mg·kg-1FDPM可显著减少NOS阳性神经元 ,其作用优于相似剂量的 1,6 二磷酸果糖 (FDP)和硫酸镁 ,而 133mg·kg-1的FDPM没有明显作用。结论 :脑出血后NOS活性变化呈双峰现象 ;

AIM: To study the timed number of nitric oxide synthase (NOS) positive cells and the effects of sodium magnesium fructose diphophate (FDPM). METHODS: Rat model of intracerebral hemorrhage (ICH) was induced by collagenase, the timed number of NOS positive cells and the effects of FDPM on it was determined by NADPH histochemical staining. RESULTS: After the onset of ICH, the number of NOS positive cells increased obviously at 2 hours, decreased lightly at 6 hours, increased again at 12 hours, peaked during from 24 hours to 3 days, and decreased again at 7 days, but was till higher than that of normal condition. Application of FDPM decreased significantly the number of NOS positive cells after ICH, and the effect of FDPM was superior to that of fructose 1,6 diphophate or magnesium sulfate. CONCLUSION: The change of NOS activity shows two peaks after ICH, and inhibition of NOS activity may be one of the important protective mechanisms of FDPM from ICH.