RAGE基因Gly82Ser多态性与脑梗死的相关性研究

A study on the association of RAGE gene Gly82Ser polymorphism with cerebral infarction patients

目的针对脑梗死多因素致病的特点,探讨RAGE基因Gly82Ser多态性与糖尿病合并脑梗死、高血压合并脑梗死和单纯脑梗死的关系。方法应用聚合酶链反应-限制性片段多态性(PCR-RLFP)的方法检测糖尿病合并脑梗死56例,高血压合并脑梗死60例,单纯性脑梗死46例,健康人对照50例汉族人RAGE基因Gly82Ser外显子3内82位点基因型频率和等位基因频率。结果高血压合并脑梗死组病人RAGE基因杂合子基因型(GS)频率明显高于对照组(P<0.05),其等位基因(G,S)频率与对照组比较无差异(P>0.05);糖尿病合并脑梗死组和单纯脑梗死组基因型(GG,GS,SS)和等位基因(G,S)与对照组比较无统计学差异(P>0.05)。结论高血压合并脑梗死病人RAGE基因Gly82Ser杂合子基因型(GS)增多,可能是高血压合并脑梗死病人的危险因素;RAGE基因Gly82Ser的多态性与糖尿病合并脑梗死和单纯脑梗塞病人发病、发展无关。

To study the relationship of RAGE gene Gly82Ser polymorphism with diabetes combined, hypertension combined and simply cerebral infarction according to multiple factors and multiple genes leading to disease. Methods The Gly82Ser gene at position of RAGE gene exon 3 was determined by a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method in 56 cases of diabetes mellitus combined with cerebral infarction (DM&CI), 60 of hypertension combined with cerebral infarction patients (HP&CI), 46 of simply cerebral infarction patients (CI) and 50 healthy control subjects of Han nationality. Results The frequencies of RAGE gene Gly82Ser GS heterozygous genotype were significantly higher in HP&CI patients than those in control subjects (p<0.05).There was no striking difference of the GS heterozygous genotype and the S allele frequency in DM&CI group and simply cerebral infarction patients (p>0.05). Conclusion RAGE gene Gly82Ser GS heterozygous genotype may be associated with CI&HP patients. RAGE gene Gly82Ser polymorphism is not associated with DM&CI and simply CI patients.