生育期金雀异黄素干预对亲代大鼠生育能力的影响

Effect of genistein on fertility of parental rats in the period of mating

目的探讨生育期金雀异黄素干预对亲代大鼠体重及生育能力的影响。方法 SPF级性成熟8周龄雌雄SD大鼠实验室适应1周后,随机分为4组,分别为高剂量组、中剂量组、低剂量组以及对照组,每组雌鼠8只,雄鼠4只。按雌雄比例为2∶1进行合笼交配,同时给予含有相应剂量的金雀异黄素的高脂饲料干预3周。其中,低、中、高剂量金雀异黄素组每千克饲料分别加入金雀异黄素600、1800、5400 mg,对照组不添加金雀异黄素。实验期间每周称量体重,实验结束后摘取脏器称重,观察雄性大鼠精子密度及母鼠怀孕率。结果与对照组比较,高剂量金雀异黄素可以降低母鼠及父鼠体重,差异有统计学意义(P<0.05)。同时高剂量金雀异黄素可以降低雄性大鼠精子密度,降低母鼠受孕率。结论生育期金雀异黄素干预可以显著降低亲代大鼠的生育能力。

Objective To explore the effect of genistein on body weight and fertility of parental rats in the period of mating. Methods After adaption for 1 week, 8 weeks female and male SD rats were randomized to 4 groups: high-dose group, middle-dose group, low-dose group and control group, 8 female rats and 4 male rats in each group. Female and male rats were mating at ratio of 2∶1. Different dosage of genistein were given at the beginning of mating fed with highfat diet for 3 weeks. The high, meddle and low dose groups were given 5400, 1800, 600 mg genistein into every kg fodder diet respectively. No genistein was added in control group. Body weight was weighted every week. The organ weights of male and female rats were weighted, the sperm density of male rats and pragnancy rate of female rats were observed at the end of experiment. Results Compared with control group, body weight of female rats in high-dose group decreased, the difference was statistically significant(P < 0.05). Meanwhile, the sperm density of male rats and pregnancy rate of female rats reduced in high-dose genistein group. Conlusion Genistein can reduce fertility of parental rats in the period of mating.