基于线粒体功能进行抗炎蒙药筛选和机制研究的设想

Screening of Mongolian anti-inflammatory drugs and its working mechanism study based upon the mitochondrial function

蒙医药学是具有鲜明的民族特色、地域特点和独特理论体系、临床特点的我国民族传统医药学。在抗炎方面,蒙药具有确切的疗效和独特的作用。但很多抗炎蒙药的作用机制都缺少现代科学方法和技术的深度分析和加工升华。为了抗炎蒙药的市场化和进一步开发利用,需要探索出一个新的研究思路和方法。线粒体是细胞中提供能量的重要细胞器,人体多种代谢性和免疫相关的疾病都与线粒体的功能异常密切相关,从微观系统的角度理解线粒体是影响人体生理状况的重要影响因子。采用系统中的一个重要元素,反映整个系统的状态以及系统对外界刺激的应答反应是一个非常重要的研究方法,它与蒙医的整体论思想是非常契合的。UCP2和PGC-1α是有效反映线粒体的两个功能蛋白。本文阐述了通过检测以上两个蛋白的表达和活性来探讨线粒体功能的状态,甚至是细胞、组织的情况,借此提出了研究抗炎蒙药的作用机制和和筛选活性成分的新研究思路和方法。

As a traditional national medication, Mongolian medication has unique characteristics of national, geographical, theoretical and clinical characteristics. Mongolian drug has particular effect on anti-inflammation. However, the anti-inflammation mechanism of a variety of drugs is not clear due to limited modern science methodologies and techniques. In order to develop Mongolian anti-inflammation drug market and exploit its utilization, urgently require to find out an innovative research strategy and methodology. Mitochondria, an important organelle for cellular energy transduction, has been functionally linked to many kinds of metabolic and immunological diseases. From micro-systematic viewpoint, mitochondria are crucial factors for human physiological status. Digging into this factor through its interaction with the whole system, and also its response to external stimulation is always an important direction for research.And this theory is perfectly agreed with the idea of Mongolian medication. Uncoupling protein 2(UCP2) and peroxisome proliferator-activated receptor-γcoactivator-1α(PGC-1α) are two important functional indicators for mitochondria. In this study, the expression and activity of both UCP2 and PGC-1α are analyzed to evaluate the mitochondrial status, and so as to check whether cells or tissues were normally functioning. Based on this study, a generalized research methodology for studying the physiology and selective active ingredients of Mongolian anti-inflammation drugs is presented and proved.