宽场光学相干断层成像系统的三维显微成像

3D microscopic imaging based on wide-field optical coherence tomography

宽场相干断层成像技术(WFOCT)具有提高OCT系统的扫描速率和实现高分辨率的三维显微技术等优点,成为当前研究的热点。本文阐述了WFOCT的基本原理,利用八步移相法重建出玻璃物体微细结构的断层图像,研究了宽场OCT系统对玻璃材料的纵向分辨率和探测深度,其中探测深度可达3.3mm。在获得多幅断层图像的基础上,利用VC6.0和OpenGL混合编程,采用移动立方体(MC)算法重建出玻璃物体微细结构的三维图像。实验结果表明,WFOCT系统不但能够在生物组织检测等医学方面得到应用,而且对反射率较高的物体能够完成三维形貌显微成像探测和深度探测。

Wide-field optical coherence tomography(WFOCT)has a promising application for its high scanning rate and resolution.The principle of wide-field optical coherence tomography was described,and 2Dimages of glassware were reconstructed using eight-step phase-shifting method.Besides,the axial resolution and depth detection were studied,and the depth detection could reach 3.3mm in view of glass material.After obtaining several 2Dimages,by using VC6.0and OpenGL programming,the 3Dmicroscopic imaging was realized based on the marching cube(MC)algorithm.The experimental results show that the WFOCT system can be applied in the biological tissues detection as well as the 3D morphology microscopic imaging and depth detection for the objects of high reflectivity.