显微镜景深拓展技术研究

Extended depth of field in microscopy

随着生命科学需求的日益迫切,显微镜的景深拓展成为显微领域的一个重要发展方向,为了更好地了解、研究显微景深问题,对景深拓展技术进行研究与总结。以显微景深的大幅提升为出发点,就光学切片扫描、空间光调制、波前编码及光场显微4个方面进行了全面的综合性论述。对这些技术的原理、方式及拓展水平进行了阐述,并就其技术缺陷、发展方向进行了归纳总结。随着技术水平的提高,显微景深较传统显微镜可提高1~2个数量级。

Due to the urgent needs in life sciences ,the extended depth of field (EDOF) in mi‐croscopy is becoming a focus .In order to study the microscopy depth of filed ,the EDOF was studied and summarized .Based on the dramatic enhancement of EDOF ,the optical scanning , spatial light modulation ,wavefront coding and light filed microscopy were introduced and com‐pared synthetically .Their theory ,method and capacities for EDOF were summarized ,and the technology defect and future development were analyzed .With the enhancement of technolo‐gies ,the depth of filed in microscopy can be enhanced about 1 or 2 orders of magnitude com‐pared with traditional microscopy .