金丝桃素提取物稳定性考察

Stability of Hypericin Extract

目的考察金丝桃素提取物的稳定性。方法以金丝桃素和伪金丝桃素含量变化为指标,考察光照、温度、抗坏血酸等因素对金丝桃素提取物短、长期稳定性的影响。结果短期内,金丝桃素提取物在常温避光及加抗坏血酸条件下较稳定;光照能使提取物中金丝桃素、伪金丝桃素含量短时间内升高,然后开始下降;在高温条件下,提取物稳定性差。8周内,提取物在常温光照、常温避光及加抗坏血酸条件下金丝桃素、伪金丝桃素含量均有不同程度地下降,在低温避光条件下比较稳定。结论金丝桃素提取物应低温、避光保存。

Objective To investigate the stability of hypericin extract. Methods Hypericin and pseudo-hypericin were detected by high performance liquid chromatography ( HPLC ) . The content change of hypericin and pseudohypericin in hypericin extract was used as the indicator. The effects of light, temperature and ascorbic acid on the short-term and long-term stability of hypericin extract were stud-ied. Results In the short term, the hypericin extract was more stable under the conditions of the room temperature away from light and adding ascorbic acid; the hypericin and pseudo-hypericin contents were increased within a short time and then began to decline in light;the extract stability was poor at the high temperature. Within 8 weeks, the hypericin and pseudo-hypericin contents in the hyper-icin extract were declined in varying degrees under the conditions of light at the room temperature, protected from light at the room temperature and adding ascorbic acid;but which were comparatively stable under the low temperature without light. Conclusion The hypericin extract should be stored at the low temperature without light.