阿司匹林抑制去势大鼠IL-1,IL-6,TNF-α和M-CSF的表达及其防治绝经后骨质疏松作用的研究

Aspirin in Restraining IL- 1,IL- 6,TNF- α and M- CSF Expression and Its Effect in Preventing Post- Menopause Osteoporosis

目的探讨阿司匹林对去卵巢骨质疏松模型小鼠体内白细胞介素-1(IL-1)、IL-6、肿瘤坏死因子-α(TNF-α)及巨噬细胞集落刺激因子(M-CSF)表达的影响,以及其防治骨质疏松的作用。方法选取Sprague-Dawley大鼠60只,随机分为对照组(10只)和去卵巢模型组(50只),术后1周去卵巢模型组大鼠再随机分为骨质疏松组,其中,A组(0.25 mmol/L阿司匹林)、B组(0.5 mmol/L)、C组(1.0 mmol/L)和D组(1.5 mmol/L),各10只。检测各组血清和骨组织IL-1,IL-6,TNF-α和M-CSF水平,同时采用Micro-CT对骨小梁微观三维形态结构进行分析。结果骨质疏松组血清IL-1,IL-6,TNF-α,M-CSF分别为(80.15±7.18)pg/m L、(82.16±6.63)pg/m L、(30.21±4.73)pg/m L和(11.42±4.25)pg/m L,均明显高于对照组(P<0.05)。A,B,C,D组中,D组IL-1,IL-6,TNF-α和M-CSF水平最低(P<0.05)。骨质疏松组骨组织IL-1,IL-6,TNF-α和M-CSF分别为(71.24±8.10)pg/m L、(78.02±5.15)pg/m L、(26.92±3.23)pg/m L和(10.22±3.15)pg/m L,均明显高于对照组(P<0.05);A,B,C,D组中,D组IL-1,IL-6,TNF-α和M-CSF水平最低(P<0.05)。Micro-CT检测结果表明,阿司匹林治疗组腰椎椎体骨小梁厚度、骨小梁数量及骨密度均显著高于骨质疏松组。结论阿司匹林能抑制去势大鼠骨流失,这可能与阿司匹林抑制大鼠血清和骨组织IL-1,IL-6,TNF-α和M-CSF的表达有关,从而使破骨活动减弱的作用。

Objective To investigate the influence of aspirin on ovariectomized osteoporosis in mice interleukin-1 ( IL-1 ) , interleukin-6 ( IL-6 ) , tumor necrosis factor ( TNF-α) and macrophage colony-stimulating factor ( M-CSF ) expression and its role in prevention and treatment of osteoporosis. Methods 60 Sprague-Dawley rats were randomly divided into sham operation group ( control group, 10 rats ) and ovariectomized model group ( 50 rats ) , and the ovariectomized model group were then randomly divided into the osteoporosis group, group A ( given 0. 25 mmol/L aspirin ) , group B ( given 0. 5 mmol/L aspirin ) , group C ( given 1. 0 mmol/L aspirin ) and group D ( given 1. 5 mmol/L aspirin ) at 1 week after operation, 10 rats in each group. The serum and bone tissue IL-1, IL-6, TNF-α and M-CSF level were detected, while the scanning electron microscope was used to analyze every area of bone resorption. Results The IL-6, serum IL-1, TNF-α and M-CSF of the osteoporosis group were ( 80. 15 ± 7. 18 ) pg/mL, ( 82. 16 ± 6. 63 ) pg/mL, ( 30. 21 ± 4. 73 ) pg/mL and ( 11. 42 ± 4. 25 ) pg/mL respectively, which were significantly higher than those of the control group ( P < 0. 05 );in group A, B, C, D, the IL-1, IL-6, TNF-α and M-CSF levels of group D were the lowest ( P < 0. 05 );the bone tissue IL-1, IL-6, TNF-α and M-CSF of the osteoporosis group were ( 71. 24 ± 8. 10 ) pg/mL, ( 78. 02 ± 5. 15 ) pg/mL, ( 26. 92 ± 3. 23 ) pg/mL and ( 10. 22 ± 3. 15 ) pg/mL respectively, which were significantly higher than those of the control group ( P < 0. 05 );in group A, B, C, D, the IL-1, IL-6, TNF-α and M-CSF levels of group D were the lowest ( P < 0. 05 );the micro -CT analysis showed that the vertebral trabecular thickness, trabecular bone volume and bone mineral density of the aspirin group were significantly higher than those of the OVX group. Conclusion Aspirin can inhibit the serum and bone tissue of ovariectomized osteoporosis mice model IL-1, IL-6, TNF-α and M-CSF expression, weaken the osteoclast activity, and to prevent osteoporosis.