反相高效液相色谱法同时测定正柴胡饮合剂中橙皮苷和芍药苷含量

Simultaneous Determination of Hesperidin and Paeoniflor in Radix Bupleuri Decoction by RP- HPLC

目的建立同时测定正柴胡饮合剂中橙皮苷和芍药苷含量的反相高效液相色谱(RP-HPLC)法。方法采用超声提取的方法,色谱柱为phenomenex-ODS柱(250 mm×4.60 mm,5μm),柱温为30~35℃,流动相为乙腈-1 mol/L磷酸溶液(15∶85),橙皮苷和芍药苷的检测波长分别为283 nm和230 nm,流速为0.8~1.0 m L/min。结果橙皮苷进样量在0.021 99~4.420μg(r=0.999 9)范围内与峰面积线性关系良好,平均回收率为95.00%,RSD为4.64%;芍药进样量在0.201 0~1.002 3μg(r=0.999 9)范围内与峰面积线性关系良好,平均回收率为98.04%,RSD为3.60%。结论该法操作简便,检测灵敏,结果准确,对于正柴胡饮合剂的质量控制具有非常重要的作用和意义。

Objective To establish the simultaneous determination of hesperidin and paeoniflorin in Radix Bupleuri Decoction by RP-HPLC. Method By using the method of ultrasonic extraction, and the phenomenex-ODS chromatographic column ( 250 mm × 4. 60 mm, 5 μm ) , the column temperature was 30-35 ℃, the mobile phase was -1 mL/L phosphoric acid solution ( 15: 85 ) , the detection wavelength of hesperidin and paeoniflorin were 283 nm and 230 nm, and the flow rate was 0. 8-1. 0 mL/min. Results In 0. 021 99-4. 420 μg ( r=0. 999 9 ) range, the linear relationship of hesperidin was good, the average recovery was 95. 00% , RSD was 4. 64% ; in 0. 201 0-1. 002 3 μg ( r=0. 999 9 ) range, the linear relationship of paeoniflorin was good, the average recovery was 98. 04%, RSD was 3. 60%. Conclusion This method has the advantages of simple operation, sensitive detection, accurate results, and has an important role and significance of the quality control of Radix Bupleuri Decoction.