高效液相色谱法测定二甲散结胶囊中丹参酮Ⅱ_A含量

Determination of Tanshinone Ⅱ_A in Erjiasanjie Capsules by HPLC

目的建立测定二甲散结胶囊中丹参酮ⅡA含量的高效液相色谱(HPLC)法。方法采用Shim-pack VP-ODS C18柱(150 mm×4.6mm,5μm),甲醇-水(75∶25)为流动相,流速为1.0 m L/min,检测波长为270 nm。结果丹参酮ⅡA质量浓度在2.472~49.44μg/m L范围内与峰面积积分值线性关系良好(r=0.999 7),平均回收率为98.15%,RSD=1.34%(n=6)。结论该方法准确、简便,重复性和稳定性良好,适用于二甲散结胶囊的含量测定。

Objective To establish an HPLC method for the determination of Tanshinone ⅡAin Erjiasanjie Capsules. Methods The chromatographic system consisted of Shim- pack VP- ODS C18 column( 150 mm × 4. 6 mm,5 μm),using methanol- water( 75 ∶ 25) as mobile phase. The content was detected at a wavelength of 270 nm,the flow rate was 1. 0m L / min. Results The Tanshinone ⅡAhave a good tincar rolatim in the range of 2. 472- 49. 44 μg / m L( r = 0. 999 7),the average recovery was 98. 15% and RSD = 1. 34%( n = 6).Conclusion The established method is accuracy,sensitive and simple. It can be used for quality control of erjiasanjie capsules.