视黄酸受体在脐血淋巴细胞细胞因子表达中的调节作用

Regulation of retinoic acid receptor on cytokine expressions in cord blood lymphocytes

目的研究视黄酸(RA)促进脐血淋巴细胞(CBL)细胞因子表达的作用途径,探讨视黄酸受体α在该过程中的可能作用。方法收集19例健康新生儿的脐血标本进行体外培养,采用 RT-PCR 法检测 CBL 中视黄酸受体α(RAR-α)、IL-2、IFN-γ和 IL-4的 mRNA 水平。结果 RA 上调 CBL 中 RAR-α的基因表达水平,培养24h 时的上调幅度为(1.17±0.07)倍(P<0.001)。RA 上调 IL-2的基因表达水平,培养24h 时的上调幅度为(1.29±0.20)倍(P<0.001)。RA 也上调IFN-γ的基因表达水平,培养48h 时的上调幅度为(1.39±0.26)倍(P<0.001)。RA 还上调 IL-4的基因表达水平,培养24h 时的上调幅度为(1.52±0.44)倍(P<0.002)。RA 使 RAR-α基因表达的上调幅度与 RA 使 IL-2、IFN-γ和 IL-4基因表达的上调幅度皆存在正相关,r 值分别为0.40、0.39和0.46,(P<0.05)。结论 RAR-α可能参与 RA 对 CBL 细胞因子(IL-2、IFN-γ和 IL-4)表达的促进作用。RA 可能通过增加 RAR-α的表达而上调 IL-2、IFN-γ及 IL-4等细胞因子的表达,从而促进 CBL 的功能。

Objective To explore the path of retinoic acid(RA)promoting cytokine expressions in cord blood lymphocytes(CBL).Methods Nineteen cord blood sanples from healthy newborns were collected and CBL were cultured in vitro.The mRNA levels of retonoic acid receptor-α(RAR-α),IL-2,IFN-γ and IL-4 were determined by reverse transcription-polymerase chain reaction(RT-PCR).Results The RAR-α expression was increased with the optimal concentration of RA,the enhanced amplitude was(1.17±0.07)times after culture for 24 hours(P<0.001). The expression of IL-2 was augmented by RA,the enhanced amplitude was(1.29±0.20)times after culture for 24 hours(P<0.001).The expression of IFN-γ was augmented by RA,the enhanced amplitude was(1.39± 0.26)times after culture for 48 hours(P<0.001).The expression of IL-4 was augmented by RA,the enhanced amplitude was(1.52±0.44)times after culture for 24 hours(P<0.002).There were positive correlations between the enhanced amplitude of RAR-α expression and the enhanced amplitudes of IL-2、IFN-γ and IL-4 expres- sions,r=0.40,0.39,0.46,respectively(P<0.05).Conclusion RAR-α might be involved in the promotive cytokine expressions in CBL by RA.