共轭亚油酸对肥胖大鼠UCP2基因表达的影响

The effect of conjugated linoleic acid on the gene expression of the UCP2 in obese rat fed with high fat diet

目的通过研究共轭亚油酸(conjugated linoleic acid,CLA)对饮食诱导肥胖大鼠解偶联蛋白2(uncoupling protein 2,UCP2)基因表达的影响,探讨CLA降低体重、体脂作用的机制。方法选用雄性Wistar大鼠,随机分为对照组、高脂组、高脂+CLA组(每100g饲料含CLA分别为0.75g、1.50g、3.00g),每组动物10只,观察CLA对肥胖大鼠体重、体脂、血脂水平的影响,并应用RT-PCR的方法检测UCP2 mRNA的表达水平。结果 CLA可降低饮食诱导肥胖大鼠的体重和体脂含量,降低血清甘油三酯、胆固醇、游离脂肪酸水平,并可增加肥胖大鼠脂肪组织UCP2 mRNA的表达水平。结论 CLA可改善肥胖大鼠的脂代谢紊乱,增加UCP2基因的表达,发挥降低体重、体脂的作用。

Objective To study the effect of conjugated linoleic acid (CLA) on expression of Uncoupling protein 2 (UCP2) in white adipose tissue of obese rats, and explore the mechanism of reduce the body weight and fat content of obeses rats by CLA. Methods Male Wistar rats were randomly separated to control group, high-fat group and high fat+CLA group (0.75g, 1.50g, 3.00g% by diet weight). We observed the effect of conjugated linoleic acid on body weight, fat content and blood lipids, Using reverse transcription polymerse chain reaction (RT-PCR) technique to measure the mRNA expression level of UCP2. Results CLA reduced the body weight, fat content, decreased serum triglyceride, cholesterol, free fatty acid levels, and increased the expression of UCP2 mRNA in adipose tissue of obese rat. Conclusions CLA can improve dyslipidaemia of obese rat and reduced weight body and fat content byincreasing the expression of UCP2 mRNA.