已酮可可碱对内毒素所致SIRS大鼠核转录因子-KB的调控

Effect of pentoxifylline on the activation of nuclear transcription factor Kappa B of rats with Systemic Inflammatory Response Syndrome induced by endotoxin.

目的:探讨已酮可可碱(pentoxifylline,PTX)对内毒素所致全身炎症反应综合征(systemic inflammatory response syndrome,SIRS)大鼠核转录因子-kB(NF-kB)的调控。方法用内毒素复制SIRS大鼠。实验分三组,即正常对照组、SIRS组和PTX组。用ELISA的方法检测各组NF-kB活性以及TNF-α和IL-1β的表达。结果与正常对照组比较,SIRS组大鼠NF-kB活性以及TNF-α和IL-1β的表达明显升高(P<0.01);与SIRS组比较,PTX组大鼠NF-kB活性以及TNF-α和IL-1β的表达明显降低(P<0.01)。在SIRS组大鼠中NF-kB活性与TNF-α和IL-1β的表达分别呈明显正相关(r=0.67,r=0.43,P<0.01)。结论PTX可明显抑制SIRS大鼠的炎症反应,其作用机制可能是抑制NF-kB的活化。

Objective: To explore the effects of pentoxifylline (PTX) on the activation of nuclear factor Kappa B (NF- κ B) of rats with systemic inflammatory response syndrome induced by endotoxin. Methods: The animal model of SIRS was established by endotoxin. 60 Wistar rats were distributed into three groups randomly. They are the control group, SIRS group and PTX group. the activation of NF- κ B and the expressions of TNF- α and IL- 1 β were examined by ELISA. Results: Compared with the control group, the activation of NF- κ B and the expressions of TNF- α and IL-1 β in the SIRS group increased significantly (P < 0.01).Compared with the SIRS group, the activation of NF-kB and the expressions of TNF-α and IL-1βin the PTX group decreased significantly (P<0.01). In the SIRS group, there are significant positive correlation between the activation of NF-κB and the expressions of TNF- α and IL-1 β respectively.( r = 0.67,r=0.43,respectively,P<0.01). Conclusion: PTX may alleviate the inflammatory reaction of SIRS by inhibiting NF-κB activation.