职业砷接触工人外周血miRNA生成和成熟相关mRNA表达变化

Changes of m RNA expression related with generation and maturation of mirna in workers exposed to arsenic

目的探讨职业砷接触工人mi RNA生成和成熟相关m RNA表达变化规律,深入认识砷的致癌机制。方法整群随机抽取正在进行砷冶炼的工人43名作为高暴露组,已经停止砷冶炼85 d的工人36名作为低暴露组,对照组24人。用实时荧光定量PCR(RT-PCR)检测外周血mi RNA生成和成熟相关m RNA表达情况,采用氢化物发生原子吸收分光光度法检测尿砷含量。尿砷浓度和相关m RNA的-△△Ct值组间比较采用t检验。检验水准α=0.05。结果高暴露组尿砷为(1738.62±1361.62)μg/g肌酐,低暴露组为(561.20±451.50)μg/g肌酐,对照组为(17.63±11.07)μg/g肌酐,各组间差异具有统计学意义(P<0.01)。高暴露组外周血mi RNA生成和成熟相关m RNA Lin28、Lin28b、Exportin-5、Trbp、Dicer、Ago2 RT-PCR扩增-△△Ct与低暴露组和对照组间差异均具有统计学意义(P<0.01),而低暴露组与对照组间差异均无统计学意义(P>0.05)。结论砷冶炼工人机体内可能存在大量mi RNA生成和成熟相关m RNA表达增加。此过程是可逆的,砷中毒患者早诊、早治非常重要。

Objective To investigate the rule of m RNA expression related with generation and maturation of mi RNA in workers exposed to arsenic, study the carcinogenic mechanism of arsenic deeply.Methods We selected 43 workers in one arsenic plant which was producing arsenic trioxide(high exposure group), and 36 workers from another plant which the production had stopped 85 days(low exposure group) by random cluster sampling. RT-PCR was performed to detect the expressions of m RNAs related with generation and maturation of mi RNA. It was student's t test used to analyse the differences along groups for urinary arsenic and the-△△Ct value of relative mi RNAs. Test level was α=0.05.Results The concentration of urinary arsenic were(1738.62±1361.62)μg/g Cr in high exposed group,(561.20±451.50)μg/g Cr in low exposed group, and(17.63±11.07)μg/g Cr in control group, and there were significant difference along three group(P<0.01). The-△△Ct came from RT-PCR for Lin28,Lin28 b,Exportin-5, Trbp, Dicer and Ago2 were(9.88±2.42),(8.49±3.10),(9.57±2.36),(6.07±1.62),(6.96±1.60)and(6.91±2.02)respectively in m RNAs(P<0.01), but not between low exposed group and control group for all m RNAs(P>0.05). Conclusions There are many mi RNAs which are generating and maturating in workers produce arsenic trioxide. At the same time, the expressions of many relativem RNA increase. The process is reversible, so it is of great important to diagnosis and treat early patients.