阿尔茨海默病患者血清CD33水平及其临床意义

Analysis of the expression and diagnostic value of CD33 in Alzheimer's disease

目的探讨CD33在阿尔茨海默病(AD)中表达情况及诊断价值,为临床诊断提供参考。方法选择山东菏泽市立医院收治的73例AD患者作为AD组,30例帕金森病(PD)患者作为PD组,以及30例健康受试者作为对照组,检测并比较各组受试者血清C反应蛋白(CRP)、同型半胱氨酸(HCY)、CD33表达情况,其中CRP和HCY采用全自动生化分仪检测,CD33采用流式细胞仪检测,分析CD33与CRP、HCY相关性,并采用受试者工作特征(ROC)曲线分析CD33对AD诊断价值。结果三组受试者血清CRP和HCY水平比较,差异有统计学意义(P<0.05)。AD组患者血浆CD33+单核细胞比率明显低于PD组与对照组的,差异有统计学意义(P<0.05);而PD组与对照组CD33+表达情况比较,差异无统计学意义(P>0.05)。AD患者CD33+表达率与CRP、HCY水平呈显著正相关关系(r=0.682、0.610,P<0.05)。在PD患者中,CD33对AD诊断曲线下面积为0.771。在对照组中,CD33对AD诊断曲线下面积为0.908。结论 CD33对阿尔茨海默病患者的影响可能与炎症反应有关,CD33对AD患者具有着一定的诊断价值,可作为一种潜在的AD诊断生物标志物。

Objective To investigate and analyze the expression and diagnostic value of CD33 in Alzheimer's disease(AD). Methods Totally 73 cases of AD patients in Heze Municipal Hospital were selected as AD group, and 30 cases of Parkinson's disease(PD) were selected as PD group and30 healthy subjects were selected as control group. The serum C reactive protein(CRP), homocysteine(HCY) and CD33 levels were detected and compared among the three groups. CRP and HCY were detected by fully automated biochemical analyzer. CD33 was detected by flow cytometry. The correlation between CD33 and CRP and HCY was analyzed. The value of CD33 on diagnosing AD was analyzed by using the subjects' working characteristics(ROC) curve. Results There were significant differences of serum CRP and HCY in the three groups(P<0.05), and both CRP and HCY levels in AD group were higher than those in PD group and control group. The plasma CD33^+ positive monocyte ratio in AD group was significantly lower than that in PD group and control group(P<0.05),but there was no difference in CD33^+ expression between PD group and control group(P>0.05).There were significant positive correlations between expression of CD33^+ and levels of CRP(r=0.682,P<0.05) and HCY in AD patients(r=0.610, P<0.05). In PD patients, the area under the ROC curve for the diagnosis of AD was 0.771, and in the control group, the area was 0.908. Conclusion CD33 in AD patients may be related to inflammatory reaction. CD33 has certain diagnostic value for AD patients, so it can be used as a potential biomarker for AD diagnosis.