野生毛花猕猴桃叶片和果实AsA含量的SSR标记关联分析

Association Analysis on Leaf and Fruit AsA Content and SSR Markers of Wild Actinidia eriantha

以抗坏血酸(AsA)含量变异丰富的野生毛花猕猴桃(Actinidia eriantha Benth.)种群为试验材料,对其叶片和果实中的AsA含量与SSR标记进行关联分析。结果表明:通过SSR分子标记技术和群体结构分析,可将70份野生毛花猕猴桃种质材料分为4个亚群;GLM模型分析结果显示,在P<0.01水平上检测有7个SSR标记与叶片和果实AsA含量紧密相关,各标记对叶片和果实AsA含量变异的解释率在0.082 5～0.175 5;MLM模型分析结果显示,在P<0.01的水平上共检测到有6个SSR标记分别与叶片和果实AsA含量紧密相关,各个标记的解释率在0.073 6～0.154 6之间。发现了与叶片及果实中的AsA含量关联的优异SSR标记位点(UDK96-040、UDK96-053、UDK97-408、UDK97-414和Ke227)。

Association analysis on leaf and fruit AsA content and SSR markers were studied by using wild Actinidia eriantha Benth.populations which AsA content was rich variation as experimental material.The results indicated that by using SSR marker and genetic structure analysis showed that the population of these materials was composed of 4 subpopulations.At P<0.01 level,there were 7 SSR markers associated with the leaf and fruit AsA content under GLM program,and the rate of explanation on the phenotype of related marker ranged from 0.082 5 to 0.175 5.There were 6 SSR markers associated with the leaf and fruit AsA content under MLM program,and the rate of explanation on the phenotype of related marker ranged from 0.073 6 to 0.154 6.Found excellent SSR markers(UDK96-040,UDK96-053,UDK97-408,UDK97-414 and Ke227)associated with the leaf and fruit AsA content.