摘要
针对番茄4个抗病基因Tm-2、Pto、Sw-5和Ve1,根据其序列的碱基差异设计引物,经过引物特异性检测和PCR产物克隆测序比对之后,采用高分辨率熔解曲线(High resolution melting,HRM)技术进行多态性检测,共开发了5个SNP标记。经过验证,所开发的标记均能将抗病基因型不同的番茄材料分型,并且分型结果与已知材料的抗病性状完全一致。这些标记可以作为功能标记在番茄抗病育种中应用。
According to the gene sequence differences,primers were designed for 4 disease resistance genes in tomato,Tm-2,Pto,Sw-5 and Ve1. After checking of primer specificity and aligning of the clone sequences of the PCR products, the HRM( High resolution melting) technology was used for polymorphism detection. In this study,5 SNPs were developed,all of which could distinguish the materials with different genotypes. What's more,the genotype was completely consistent with the known gene. So they can be used as functional markers in tomato disease resistance breeding.
出处
《园艺学报》
CAS
CSCD
北大核心
2014年第10期2012-2020,共9页
Acta Horticulturae Sinica
基金
国家‘863’计划项目(2012AA100103)
国家现代农业产业技术体系建设专项资金项目(CARS-25-A-09)
农业部园艺作物生物学与种质创制重点实验室项目
关键词
番茄
抗病基因
功能标记
HRM
tomato
disease resistance gene
functional marker
HRM
