芍药‘桃花飞雪’开花衰老期间乙烯代谢生理机制的研究

The Ethylene Metabolism in Flowers of Chinese Peony ‘Taohua Feixue' During Opening and Senescence

以芍药(Paeonia lactiflora Pall.)品种‘桃花飞雪’为试材,测定了不同发育时期花枝以及花瓣的呼吸速率、乙烯释放速率、ACC含量、ACS和ACO活性的变化。结果表明:芍药切花花瓣的乙烯释放速率有明显的跃变峰,整枝花乙烯释放表现为末期上升型。花瓣与花枝呼吸速率均有明显的跃变,花瓣的跃变峰早于整枝花。花瓣的乙烯释放速率及呼吸速率均明显高于花枝,揭示花瓣是花枝产生乙烯的主要器官,花瓣的乙烯释放决定了花枝的开放与衰老进程。花瓣ACS活性与乙烯释放量的变化趋势较为一致,说明ACS是影响乙烯生物合成的限制因子。

The respiration rate,the ethylene production,the ACC content,and the ACS and ACO activities of the flowers and the petals of Chinese peony‘Taohua Feixue'during the different development stages were analyzed. The results showed that the flower of‘Taohua Feixue'belonged to the like ethylene climacteric type and the respiration climacteric type. The ethylene production of the petals had an obvious climacteric peak,while the ethylene production of the intact flower increased during later stage. It revealed that the respiration rate of the petals and the intact flower was characterized by a typical climacteric peak and the respiration rate of the petals peaked earlier. Compared to the intact flower,the ethylene production and respiration rate of the petals were higher than those of the intact flower. The results indicated that the petals were the major organ of the ethylene production,which was closely associated with the flower opening and senescence. The ACS activity was consistent with the endogenous ethylene production,which suggested that ACS was the important rate-limiting enzyme of the ethylene biosynthesis.