茄子紫外光受体蛋白基因SmUVR8的克隆及功能分析

Molecular Cloning and Functional Characterization of UV-B Photoreceptor Gene SmUVR8 in Solanum melongena

以光敏感型茄子(Solanum melongena L.)‘蓝山禾线茄’子叶为材料,克隆了紫外光受体蛋白基因UVR8同源基因SmUVR8的cDNA全长序列,并对序列进行生物信息分析,通过转化拟南芥突变体以及实时荧光定量PCR研究其功能。序列分析表明,SmUVR8的CDS全长1 530 bp,编码509个氨基酸,属于较不稳定亲水性蛋白。蛋白序列比对发现,SmUVR8与其他茄科植物的UVR8高度同源。通过拟南芥uvr8突变体转化试验发现,转基因植株(SmUVR8/uvr8)恢复了野生型的表型,说明SmUVR8与拟南芥的UVR8功能具有相似性。通过实时定量PCR发现,UV-B照射影响SmUVR8以及下游SmHY5和SmCHS的表达。酵母双杂交结果表明,SmUVR8与SmCOP1的互作依赖于UV-B。推测UV-B在茄子中是通过紫外光受体SmUVR8与SmCOP1的互作,促进SmHY5和SmCHS的表达。

Ultraviolet-B(UV-B)receptor gene UVR8 was cloned from eggplant(Solanum melongena L.)using homology-based cloning method,named SmUVR8.Sequence alignment shows SmUVR8 is similar to UVR8 in other Solanaceae plant and Arabidopsis thaliana,indicating SmUVR8 may have the function of AtUVR8.To further study the function of the SmUVR8,SmUVR8 was transformated in the Arabidopsis uvr8 mutant.The result showed SmUVR8 restored the phenotype of hypocotyl elongation,the flowering time and anthocyanin accumulation of Arabidopsis uvr8 mutant,which indicated eggplant SmUVR8 gene have the similar function to the Arabidopsis AtUVR8 gene.Real-time fluorescent quantitation and yeast two hybrid result reveals UV-B signal can influence photomorphogenesis of eggplant by the interaction of SmUVR8 and SmCOP1 activating SmHY5 and SmCHS.