竹茹多糖体外调节肠道菌群的作用研究

IN VITRO FERMENTATION OF POLYSACCHARIDES FROM BAMBOO SHAVINGS BY HUMAN GUT MICROBIOTA

目的研究竹茹多糖体外调节人体肠道菌群的作用,为竹茹多糖作为肠道微生态调节剂的研发提供理论依据。方法通过体外模拟发酵体系考察竹茹多糖对人体肠道菌群的调节作用;采用16S rDNA高通量测序技术分析肠道菌群组成,采用GC-MS法检测菌群代谢产物(短链脂肪酸)。结果竹茹多糖能够显著上调拟杆菌属、普雷沃茨菌属、双歧杆菌属、乳杆菌属、副拟杆菌属、考拉杆菌属、氨基酸球菌属、布劳特氏菌、严格厌氧梭菌属_13、Erysipelatoclostridium属、链型杆菌属、Flavonifractor属和韦荣球菌属的相对丰度,并显著下调梭杆菌属、嗜胆菌属、脱硫弧菌属、另枝菌属、Butyricimonas属、Butyricicoccus属、Eisenbergiella属、Faecalitalea属、Lachnoclostridium属、Lachnospiraceae_UCG-004属、Lachnospiraceae_UCG-008属、Lachnospiraceae_uncultured属、罕见小球菌属和土孢杆属的相对丰度;同时,竹茹多糖能够显著促进人体肠道菌群代谢产生乙酸、丙酸和丁酸。结论竹茹多糖能够显著调节人体肠道菌群,并促进其代谢产生有益于机体健康的短链脂肪酸。[营养学报,2019,41(1):45-52]

Objective To investigate the in vitro fermentation behavior of polysaccharides from bamboo shavings(named BSP)by human gut microbiota.Methods An in vitro fermentation system was used to investigate the effect of BSP on human gut microbiota.High-throughput sequencing technology(16 S rDNA)was used to analyze the composition of gut microbiota;GC-MS was used to analyze short-chain fatty acid.Results BSP could significantly modulate gut microbiota composition via increasing the relative abundances of genera Bacteroides,Prevotella7,Bifidobacterium,Lactobacillus,Parabacteroides,Phascolarctobacterium,Acidaminococcus,Blautia,Clostridiumsensu stricto13,Erysipelatoclostridium,Catenibacterium,Flavonifractor and Veillonella and decreasing the relative abundances of genera Fusobacterium,Bilophila,Desulfovibrio,Alistipes,Butyricimonas,Butyricicoccus,Eisenbergiella,Faecalitalea,Lachnoclostridium,LachnospiraceaeUCG-004,LachnospiraceaeUCG-008,Lachnospiraceaeuncultured,Subdoligran-ulum and Terrisporobacter.Meanwhile,BSP could significantly enhance the production of acetic acid,propionic acid and butyric acid.Conclusion BSP could significantly modulate human gut microbiota in vitro,and significantly enhance its production of short-chain fatty acids.[ACTA NUTRIMENTA SINICA,2019,41(1):45-52]