T-RFLP分析系统性红斑狼疮患者肠道微生物群落结构

T-RFLP Analysis of Intestinal Microflora in Patients with Systemic Lupus Erythematosus

目的:探讨本地区系统性红斑狼疮(Systemic lupus erythematosus,SLE)患者和健康人群肠道微生物菌群结构比例的差异。方法:设立健康组和SLE组,分别收集其粪便提取DNA并扩增纯化,利用末端限制性长度多样性(Terminal restriction fragment length polymorphism,T-RFLP)技术测序16S r DNA,比较两组之间的肠道微生物菌群结构比例差异。结果:通过16S r DNA进行T-RFLP测序结果显示,健康组和SLE组肠道微生物群落有所不同,SLE组厚壁菌(Firmicutes)和拟杆菌(Bacteroidetes)在微生物群落中的比例明显低于健康组。结论:系统性红斑狼疮患者与健康人群的肠道微生物群落结构存在明显差异,本研究结果为通过改变肠道微生物群落结构来预防和治疗SLE提供了相关的理论依据。

Objective Our research is to investigate the difference of intestinal microflora structure and proportion between patients with systemic lupus erythematosus(SLE)and healthy people in this region.Methods The healthy group and the SLE group were set up.Their feces were collected to extract DNA followed by DNA purification and amplification.16S rDNA sequence was determined using terminal restriction fragment length polymorphism(T-RFLP)technique,and the intestinal microflora structure and proportion were compared between the two groups.Results The 16S rDNA T-RFLP sequencing results showed that the intestinal microfloras of healthy group and SLE group were different.The proportion of Firmicutes and Bacteroidetes in the intestinal microflora of SLE group was significantly lower than that of healthy group.Conclusion There is a significant difference in intestinal microflora structure between patients with SLE and healthy people.The results of this study provide a theoretical basis for the prevention and treatment of SLE by changing the intestinal microflora structure.