摘要
Six plant growth regulators frequently used in fruit were detected using UPLC–MS/MS including chlormequat chloride, GA3, forchlorfenuron,paclobutrazol, thidiazuron and 2,4-D. A modified Qu ECh ERS method was used in pretreatment: each compound was extracted with acetonitrile containing 1% acetic acid and cleaned with a mixture of ostade-cylsilane(ODS) and Mg SO_4. The detection was performed using electrospray ionization(ESI) and multiple reaction monitoring mode(MRM) and quantified using the external standard method. Results indicated that the calibration curves of six plant growth regulators were linear within the range of 1–100 μg·kg^(-1) with correlation coefficients over 0.999. The recovery at four spiked concentrations, 1, 20, 50, and 100 μg·kg^(-1), ranged from 60.77% to 119.43% and relative standard deviation(RSD) were between 0.18% and 32.94%. The limits of detection(LOD) and limits of quantitation(LOQ) were 0.01–0.14 μg·kg^(-1) and 0.02–0.46 μg·kg^(-1), respectively, for apples, 0.01–0.18 μg·kg^(-1) and 0.03–0.59 μg·kg^(-1) for grapes, 0.01–0.08 μg·kg^(-1) and 0.04–0.28 μg·kg^(-1) for kiwis, 0.003–0.110 μg·kg^(-1) and 0.01–0.38 μg·kg^(-1) for peaches, and 0.01–0.08 μg·kg^(-1) and 0.03–0.25 μg·kg^(-1) for oranges.
Six plant growth regulators frequently used in fruit were detected using UPLC–MS/MS including chlormequat chloride, GA3, forchlorfenuron,paclobutrazol, thidiazuron and 2,4-D. A modified Qu ECh ERS method was used in pretreatment: each compound was extracted with acetonitrile containing 1% acetic acid and cleaned with a mixture of ostade-cylsilane(ODS) and Mg SO_4. The detection was performed using electrospray ionization(ESI) and multiple reaction monitoring mode(MRM) and quantified using the external standard method. Results indicated that the calibration curves of six plant growth regulators were linear within the range of 1–100 μg·kg^(-1) with correlation coefficients over 0.999. The recovery at four spiked concentrations, 1, 20, 50, and 100 μg·kg^(-1), ranged from 60.77% to 119.43% and relative standard deviation(RSD) were between 0.18% and 32.94%. The limits of detection(LOD) and limits of quantitation(LOQ) were 0.01–0.14 μg·kg^(-1) and 0.02–0.46 μg·kg^(-1), respectively, for apples, 0.01–0.18 μg·kg^(-1) and 0.03–0.59 μg·kg^(-1) for grapes, 0.01–0.08 μg·kg^(-1) and 0.04–0.28 μg·kg^(-1) for kiwis, 0.003–0.110 μg·kg^(-1) and 0.01–0.38 μg·kg^(-1) for peaches, and 0.01–0.08 μg·kg^(-1) and 0.03–0.25 μg·kg^(-1) for oranges.
基金
supported by the National Program for Quality and Safety Risk Assessment of Agricultural Products of China(GJFP2015002,GJFP2016003)
the Scientific and Technological Innovation Project of the Chinese Academy of Agricultural Sciences(CAAS-ASTIP)
