支气管哮喘患儿尿白三烯E_4的测定及其临床意义

Measurement of urinary leukotriene E_4 in asthmatic children and its clinical significance

目的 探讨支气管哮喘患儿尿白三烯E4(LTE4)测定的临床意义。方法 采用竞争性酶联免疫吸附试验技术检测 2 8例哮喘患儿急性发作期和非急性发作期的尿LTE4水平 ,并与健康组儿童相比较 ;同时对哮喘患儿的尿LTE4与第 1秒用力呼气容积 (FEV1)及外周血嗜酸性粒细胞计数 (EC)进行相关性分析。结果 哮喘患儿非急性发作期尿LTE4比急性发作期明显下降 (P <0 0 1) ,但两期均明显高于健康组儿童 (均P <0 0 1)。急性发作期尿LTE4和FEV1呈负相关 (r =- 0 6 15 ,P <0 0 1) ,和EC无相关性 (r =0 16 3,P >0 0 5 )。结论 动态检测支气管哮喘患儿尿中LTE4水平 ,可能为将来的临床诊断和治疗提供有意义的参考指标。

Objective To evaluate the clininal significance of measurement of urinary leukotrience E 4(LTE 4) in children with asthma.Methods Twenty-eight children with asthma(group one),who experinced acute attack period and asymptomatic period,and 16 controls (group two) with were measured.Urinary leukotriene E 4 in the two groups by the ACETM competitive enzyme immunoassay and expressed as pg/mg creatinine.In addition,forced expiratory volume in one second and periperal eosinophil count were investigated in the children in acute attack period.Results Urinary LTE 4 in acute attack period was significantly higher than that in asymptomatic period (geometric mean\[95%confidence interval\] 196.67(177.48～217.93) vs 171.03(153.23～190.91)pg/mg creatinine; P <0.01).Both urinary LTE 4 in acute attack period and asymptomatic period were significantly higher than the control group 76.38(66.67～87.50)pg/mg creatinine;both P <0.01).In acute attack period,there was a negative relation between urinary LTE 4 and forced expiratary volume in one second ( r =-0.615, P <0.01).There was no correlation between urinary LTE 4 and periperal eosinophil count( r =0.163, P >0.05).Conclusion Urinary LTE 4 is a useful marker of airway inflammation and can be helpful in guiding asthma management.