摘要
以美国库拉索芦荟 (Aloe .arborescens)的横切薄层切片 (transversethincelllayer,tTCL)作为转化受体 ,通过受体材料对抗生素的敏感性实验和Gus基因瞬时表达率的研究 ,找出了较适合的外植体转化条件。研究表明 :芦荟对头雹霉素 (cefotaxime)和羧苄霉素 (carbenicillin)不敏感 ,而对卡那霉素 (kanamycin)和潮霉素 (hygromycin)敏感 ;用靠近顶芽的材料得到的横切薄层切片芽再生率高 ,有较高的Gus基因瞬时表达率 ;乙酰丁香酮 (acetosyringone)在芦荟转化是不可缺少的 。
Susceptibility of the acceptors to antibiotics and transient expression of β-glucuronidase(Gus) gene were used to determine the optimized conditions for transformation of transverse thin cell layer (tTCL) of aloe(A.arborescens) mediated by Agrobacterium Tumefaciens. The results showed that both cefotaxime and carbenicillin were insusceptible while both kanamycin and hygromycin were rather sensitive to aloe. The transverse thin cell layer (tTCL) from top-part of the bud had higher percentage of shoot generation and Gus gene transient expression. Acetosyringone(AS) was necessary in aloe genetic transformation.
出处
《植物研究》
CAS
CSCD
北大核心
2004年第1期125-128,共4页
Bulletin of Botanical Research
基金
福建省自然科学基金项目
项目编号 :C9910 0 0 4
厦门凯立生物制品有限公司资助
关键词
根癌农杆菌
介导
芦荟
遗传转化
百合科
Aloe
Agrobacterium tumefaciens
genetic transformation
transverse thin cell layer
