气调包装冷鲜鹅菌群分析与鉴定

Analysis and identification of spoilage bacteria flora in modified atmosphere packaged chilled goose

将冷鲜鹅肉块以N2气调包装,置4℃冷藏4 d,序时测定其菌数变化,确定菌相构成,结果假单胞菌占41. 8%、肠杆菌占32. 7%、索丝菌占23. 1%、乳酸菌占2. 4%。代表株经染色、镜检、生理生化试验、16 S r DNA测序鉴定,结果表明:J1为莓实假单胞菌(Pseudomonas fragi)、C1为液化沙雷氏菌(Serratia liquefaciens),S1为热杀索丝菌(Brochothrix thermosphacta),R1为植物乳杆菌(Lactobacillus plantarum)。

The chilled goose was packaged in modified atmosphere adjusted with N2 in the process refrigerated of 4℃ for 4 days. Determination of the changes of bacteria and microbiological composition was condusted.The results showed that Pseudomonas account for 41. 8%,Enterobacter account for 32. 7%,Brochothrix thermosphactum account for 23. 1%,Lactic acid bacteria account for 2. 4%. The representative strain by Gram stain,microscopic examination,physiology biochemistry experiment and the method of sequencing called 16 S r DNA. The results indicated that the J1 was Pseudomonas fragi,C1 was Serratia liquefaciens S1 was Brochothrix thermosphacta,R1 was Lactobacillus plantarum.