均匀设计法优选降酶灵胶囊的提取工艺

Application of Uniform-design to optimizing condition of Jiangmeiling Capsule

目的 :优选降酶灵胶囊 (五味子 )的提取工艺。方法 :用均匀设计安排实验 ,以五味子醇甲、五味子酯甲、五味子甲素及五味子乙素含量的总和为指标 ,考察乙醇浓度、溶媒用量及冷浸时间对 4种有效成分提取率的影响 ,优选出合理的提取工艺。结果 :以 95 %乙醇为溶剂 ,提取 2次 ,乙醇用量分别为药材用量的 10倍、9倍 ,冷浸时间分别为 2 0 ,10h。提取率分别为 97.4 7% ,95 .81% ,93.77% ,95 .82 %。结论 :本工艺稳定可行 ,为降酶灵胶囊提取工艺的改进提供了依据。

Objective: To optimize the extraction process for Jiangmeiling Capsule(Fructus Schisandrae Chinensis). Methods : Uniformity design was used in extraction adoption with schisandrin, schisantherin, deoxyschisandrin, -schisandrin as marker. Results : The optimum process was that 95% alcohol used as extraction solvent, macerated twice for 20h and 10h, respectively, the amount of solvent added up to 10 and 9 times of herbs. The extraction efficiency was 97.47%, 95.81%, 93.77% and 95.82%, respectively. Conclusion : The extraction process was stable. The experimental results provided the basis for ascertainment of Jiangmeiling Capsule.