沙棘果肉油与籽油抗胃溃疡作用的比较及其机制研究

Comparative study of Sea buckthorn pulp oil with its seed oil on anti-gastric ulcer action and its mechanism

目的 :比较研究沙棘果肉油与籽油抗胃溃疡作用并对其机制进行初步探讨。方法 :采用大鼠水浸应激性、利血平型、幽门结扎型及乙酸型胃溃疡模型研究比较沙棘果肉油和籽油抗胃溃疡作用 ;以幽门结扎收集胃液观察对胃液分泌的影响 ;以甲基橙比色法观察对胃排空的影响 ;以热板法和扭体法观察其镇痛作用。结果 :沙棘果肉油和籽油对大鼠水浸应激性、利血平型、幽门结扎型胃溃疡均有明显的保护作用 ;对大鼠乙酸型胃溃疡均有明显的促进愈合作用 ;能抑制大鼠胃酸及胃蛋白酶的分泌 ,促进胃粘液的分泌 ;能抑制正常小鼠胃排空 ;可减少小鼠的扭体次数。结论 :沙棘果肉油和籽油均有抗实验性胃溃疡的作用 ,两者作用强度相似。该作用可能与其抑制胃液分泌 ,促进胃粘液分泌 ,抑制胃平滑肌运动及镇痛作用有关。

Objective: To compare anti-gastric ulcer action of sea buckthorn pulp oil(SBPO) with sea buckthorn seed oil(SBSO) and to explore its mechanism. Methods : The anti-gastric ulcer actions of SBPO and SBSO were observed and compared on the gastric ulcer induced by water immersion stress, reserpine, pylorus ligation and acetic acid. The effects on gastric secretion in rats were studied with pylorus ligation. The effects on gastric emptying were observed by methyl orange remaining rate in stomach of mouse. The analgesic effect was tested by the hot-plate method and writhing method. Results : SBPO and SBSO markedly inhibited gastric ulcer induced by water immersion stress, reserpine and pylorus ligation and promoted the healing of the gastric ulcer induced by acetic acid. SBPO and SBSO significantly inhibited the secretion of gastric acid and pepsin and promoted the secretion of gastric mucous. SBPO and SBSO delayed gastric emptying in normal mouse. SBPO and SBSO significantly decreased the number of twisting body in mice. Conclusion : Both SBPO and SBSO could have anti-gastric ulcer effect with similar potency. The mechanism of anti-gastric ulcer might be their effect of inhibition of the secrection of gastric juice, promotion of the secrection of gastric mucous, inhibition of activity of gastric smooth muscle and analgetic effect.