盐地碱蓬幼苗和种子氯仿/甲醇提取物的化学组成及其抗炎作用(英文)

Chemical composition and anti-inflammation of methanol/chloroform extracts from seeds and seedlings of Suaeda salsa (L.) Pall

目的 :阐明盐地碱蓬幼苗和种子氯仿 /甲醇提取物的化学组成及其抗炎作用。方法 :盐地碱蓬幼苗和种子分别用氯仿 /甲醇 (2∶1)提取并用甲醇 /氯乙酰 (5∶1)甲酯化。用GC MS分析甲酰化产物的化学组成。提取物对急性炎症的抑制作用采用巴豆油引起的正常小鼠和摘除肾上腺小鼠的耳肿胀、组胺引起的小鼠毛细管通透性以及角叉菜胶引起的大鼠足肿胀进行分析。对慢性炎症的抑制作用以巴豆油诱导的大鼠肉芽肿和福氏完全佐剂诱导的多发性关节炎进行分析。结果 :盐地碱蓬幼苗提取物的甲酰化产物中共检测出 14种脂肪酸甲酯 ,其中十六碳酸甲酯 (2 5 .9% ) ,9,12 十八碳二烯酸甲酯 (2 8.3% )及 9,12 ,15 十八碳三烯酸甲酯 (8.95 % )为主要成分。种子提取物的甲酰化产物中共检测出 10种脂肪酸甲酯 ,其中 9,12 十八碳二烯酸甲酯 (83.4 1% )为主要成分。两种提取物的甲酯化产物明显地抑制正常小鼠和摘除肾上腺小鼠的耳肿胀、降低毛细血管的通透性并抑制大鼠肉芽肿的形成 ,在 5 0～ 30 0mg·kg-1·d-1.剂量范围之内呈明显的剂量依赖关系 ,在 30 0mg·kg-1·d-1对佐剂性关节炎和大鼠足肿胀表现出显著的抑制作用。在 5 0～ 30 0mg·kg-1·d-1.剂量范围之内能显著地抑制炎症部位的PGE2 和MDA的形成和增加红细胞CAT的活力。?

Objective: To elucidate the chemical constituents and anti-inflammatory activity of the methanol/chloroform extracts from seeds and seedlings of Suaeda salsa (L.) Pall. Methods : The seedlings and seeds of Suaeda salsa were extracted with methanol/chloroform (2∶1) and transmethylated by methanol/acetyl chloride (5∶1). The constituents of the transmethylated extracts were analyzed by GC-MS. The ear edema of the normal and adrenalectomized mice induced by croton oil, vascular permeability formed by histamine in mice and hind paw edema caused by Carrageenin in rats were used to determine the inhibition to acute inflammnation. The suppression to the granuloma induced by croton oil and paw edema of adjuvant-induced polyarthritis in rats were utilized to verify the inhibition to the chronic inflammation. Results : Fourteen fatty acid methylesters together with four sterols in the extracts of seedlings were present and hexadecanoic acid methyl ester (25.9%), 9, 12-octadecandienoic acid methyl ester (28.3%) and 9,12,15-octadecatrienoic acid methyl ester (8.95%) were identified as the major components. Ten fatty acids methyl esters predominated by 9,12-octadecadienoic acid methyl ester (83.41%) were present in the extracts of seeds. The two extracts evidently inhibited the ear swelling of normal and adrenalectomized mice, decreased vascular permeability and suppressed the formation of granuloma in dose-dependent activity within 50-300mg·kg -1 ·d -1 . The two extracts at 300 mg·kg -1 ·d -1 suggested significant inhibition to the adjuvant-induced and Carrageenin-induced paw edema and indicated evident reduction to the formation of PGE 2 and MDA in the inflammatory paw and remarkably increased the CAT activity of erythrocytes within 50-300mg·kg -1 ·d -1 . No obvious difference was observed in the inhibition of ear swelling between the normal and adrenalectomized mice, in the weight of adrenal and vitamin C in it in different dose treatment of the test rats. The extracts from the seedlings exhibited stronger anti-inflammatory activity than that of the extracts from the seeds. Conclusion : The anti-inflammatory activity of the two extracts was mainly realized by suppressing formation of inflammatory mediators, decreasing the content of MDA from inflammatory exudates and accordingly increasing the activity of catalase. The content of gammalinolenic acid methyl ester in the extracts of seedling endowed its stronger anti-inflammatory activity than that of the extracts from seeds.