肝细胞癌抗原基因在肝细胞癌组织中的表达及临床意义

Expression and clinical significance of hepatocellular cancer antigen genes in human hepatocellular carcinoma

目的 探讨肝细胞癌抗原 (HCA)基因mRNA在肝细胞癌 (HCC)组织中的表达。方法 用逆转录 聚合酶链反应 (RT PCR)方法对HCC患者的癌组织和相应癌旁组织及肝硬化和正常肝组织中的 4种HCAmRNA的表达进行检测 ;结合临床病理资料进行分析。 结果 46例HCC患者中 ,HCA90、HCA51 9、HCA52 0、HCA587基因mRNA阳性率分别是 65 2 %、76 1 %、45 7%和 32 6 % ;至少表达一种HCA者达 82 6 % ;而癌旁组织中仅HCA51 9基因有 6 5 %mRNA表达为阳性。 1 0例肝硬化和正常肝组织中 4种HCA基因均不表达。 4种HCA的表达与患者年龄、性别、肿瘤大小、分化程度、血清AFP水平、HBV和HCV感染无显著相关性 (P >0 0 5)。在部分AFP正常的患者中存在HCA基因的表达。 结论 HCA基因在HCC中呈高比例、高特异性表达 。

Objective To investigate the expression of four hapatocellular cancer antigen (HCA) gene mRNA in hepatocellular carcinoma. Methods The expression of HCA90?HCA519?HCA520?HCA587 mRNA was detected using RT PCR in HCC tissues and the corresponding adjacent non HCC tissues from 46 HCC patients, cirrhosis tissues from 10 samples and normal liver tissues from 10 samples. The relationship between positive expression rate of HCA gene and clinical and lab data was evaluated. Results Of 46 HCC tissues, HCA90?HCA519?HCA520 and HCA587 mRNA were detectable in 65 2%?76 1%%?45 7% and 32 6%, respectively. At least one HCA gene mRNA was positive in 82 6% of HCC tissues. Only weak expression of HCA519 could be detectable in 6 5% of the corresponding adjacent non HCC tissues. None of 10 samples of cirrhosis and normal liver tissues expressed any HCA gene mRNA. No correlation was found between the expression of HCA and clinical date such as age, sex, tumor size, tumor differentiation, serum α fetoprotein level and hepatitis B virus infection or hepatitis C virus infection ( P >0 05). However, in some patients with normal serum α fetoprotein (<25 ng/L), specific expression of HCA genes was observed. Conclusion HCA gene mRNA is expressed with a high percentage and specificity in hepatocellular carcinomas and their products are new potential promising targets for immunotherapy of HCC.