银杏提取物对新生大鼠缺氧缺血性肝损伤的治疗作用

The Therapeutic Effect of Ginkgo Biloba Extract on Hypoxic Ischemic Hepatic Damage of Neonatal Rats

目的 评价银杏提取物 (extract of ginkgo biloba,EGB)对新生大鼠缺氧缺血性肝损伤的治疗作用。 方法 将 4 6只 SD新生大鼠分为 4组 :缺氧缺血组 (n=30 ) ,EGB治疗组 (n=2 4 ) ,生理盐水组 ,假手术组 (n=6 ) ,建立新生大鼠缺氧缺血性脑损伤 (HIBD)模型 ,再给氧后 2、2 4、4 8、72、16 8h后分别进行免疫组织化学及组织 HE染色 ,观察不同时间肝组织粘附分子 1(ICAM- 1)的表达、中性粒细胞浸润和肝细胞的形态学变化 ,并腹腔注射梯度剂量的 EGB治疗 ,以生理盐水和段手术作对照。 结果 肝微血管内皮细胞 ICAM- 1的表达于再给氧后 2 4 h免疫反应开始增加 [(5 2 .5±17.0 ) % ],4 8h达高峰 [(76 .5± 11.9) % ](H =2 3.9,P<0 .0 1) ,16 8h恢复正常 [(6 .9± 4 .3) % ],同时中性粒细胞浸润也随之增加 ,在时程上与 ICAM- 1表达同步。 EGB组以 5 0 mg/ (kg· d)的剂量为最佳 ,当再给氧 4 8h后 ICAM- 1免疫阳性内皮细胞数 [(17.5± 14 .9) % ]及中性粒细胞浸润比同时间缺氧缺血组明显降低、肝细胞损伤减轻 (H =2 0 .8,P<0 .0 1;D=87,P<0 .0 5 )。 结论 ICAM- 1与缺氧缺血后肝组织中性粒细胞浸润密切相关 ,5 0 m g/ (kg· d)的 EGB对新生大鼠缺氧缺血性肝损伤可能有一定保护作用。

Objective To evaluate the therapeutic effect of extract of ginkgo biloba (EGB) on hepatic damage of neonatal rats with hypoxic ischemic brain damage(HIBD). Methods Sixty six Sprague Dawley(SD) neonatal rats were divided into four groups: groupⅠincluded neonatal rats with hypoxic ischemic hepatic damage after HIBD group I, ( n=30 ), groupⅡ( n=24 ) was those with EGB-treated, groupⅢ ( n=6 ) was those with saline-treated and groupⅣ ( n=6 ) which had sham operation. ICAM-1 of liver was detected at different times in the first two groups after HIBD and the rest groups by immunohistochemical ABC method. Simultaneously HE was employed to display neutrophil infiltrations in hepatic tissue and pathologic characteristics of liver cells. Results The expression of ICAM-1 increased at 24 h [(52.5±17.0)%] with resupply oxygen, peaked at 48 h [ (76.5 ±11.9)%] ( H=23.9,P <0.01) and approached normal level at 168 h [(6.9±4.3)%] after HIBD. Neutrophil infiltrations in ischemic hepatic tissue also increased and paralleled the increase of ICAM-1 expression. The expression of ICAM-1 [(17.5±14.9)%]and neutrophil infiltration in groupⅡ with 50 mg/(kg·d) EGB decreased more significantly than that in groupⅠ at 48 h after oxygen resupply ( H=20.8,P<0.01; D=87,P<0.05 ). Conclusions These data indicated that the expression of ICAM-1 was significantly correlated with the degree of neutrophil infiltration in hypoxic ischemic hepatic tissue, and 50 mg/(kg·d) EGB might protect the hepatocytes from hypoxic ischemic damage.