THH碱不通过钙离子信号通道诱导Jurkat淋巴瘤细胞株凋亡

Role of [Ca^(2+)]i in the apoptosis of Jurkat T lymphoma cells induced by Tripterygium Hypoglaucum (Levl) Hutch alkaloids

目的 探讨昆明山海棠碱在凋亡中对细胞内游离钙离子浓度的影响。方法 THH碱诱导Jurkat细胞后 ,采用Indo 1AM及PI进行细胞染色、流式细胞术等手段在单细胞水平上检测细胞内钙离子浓度的变化。结果 THH碱诱导后细胞内钙离子浓度未见明显变化。结论 THH碱不通过钙离子信号通道诱导Jurkat细胞凋亡。

Objective To explore the roles of [Ca 2+ ]i in the apoptosis of Jurkat cells induced by Tripterygium Hypoglaucum (Levl) Hutch alkaloids. Methods After Jurkat cells were stained with Indo 1 AM and PI, changes of [Ca 2+ ]i were assessed by flow cytometry at the single cell level. Results THH alkaloids could induce no changes of [Ca 2+ ] inside cytoplasm. Conclusion THH alkaloids induce the apoptosis of Jurkat cells via other pathways rather than via the [Ca 2+ ] pathway.