犬异体气管移植的实验研究

Experimental study of canine tracheal allotransplantation

目的 通过研究与移植气管狭窄相关的因素 ,寻求可行的解决移植气管狭窄的方法。方法 16只杂种犬随机分为A、B 2组 ,每组 8只 ,行原位气管移植 ,带血管蒂大网膜包绕。A组犬术后不给予特殊处理 ,B组犬术后给予免疫抑制剂。术后 4周处死动物 ,行大体及病理学检查 ,并测定移植气管的通畅度 ,组织学半定量测定上皮及软骨情况。 结果 B组犬移植气管结构保持较好、狭窄程度低、上皮及软骨积分均较A组高 ,并且 2组犬移植体的通畅度与上皮及软骨积分均成正相关。结论 应用免疫抑制剂有助于移植体结构的保持。上皮的再生及软骨的保持与气管移植体的狭窄密切相关。

Objectives To detect the factors relavant to stenosis of tracheal graft and to find feasible methods to solve this problem. Methods Sixteen mongrel dogs were divided into groups A and B randomly and equally. Five-ring-length tracheal segments were allotransplanted. All grafts and anastomotic sites were covered with omental pedicles. In group A, no immunosuppressant was given and in group B, the recipients were treated with cyclosporine. The animals were sacrificed 4 weeks after operation, and their postmortem specimens were examined grossly and histologically. All allografts were assessed by percent patency. Epithelial regeneration and morphology of the cartilage were semiquantitatively evaluated. Results Structural integrity of the allografts were maintained better in group B than in group A. Tracheal stenosis was found to be more serious in group A. The scores of epithelial regeneration and cartilage morphology were higher in group B than in group A, and in each group positive correlation was found between the percent patency and the score of epithelial regeneration or cartilage morphology. Conclusions Immunosuppressive drugs are necessary to maintain the structure of allografts. Tracheal stenosis is correlated closely with epithelial regeneration and morphological maintenance of the cartilage.