大鼠脾脏P物质免疫反应神经纤维的分布及烫伤后含量变化

Distribution and content change of SP immunoreactive nerve fibers in the rat spleen post burn

目的 :研究P物质 (substanceP ,SP)免疫反应阳性神经纤维在大鼠脾脏的分布及烫伤后含量变化 ,探讨SP与免疫功能的关系 .方法 :用ABC免疫组织化学法结合葡萄糖氧化酶 DAB 硫酸镍胺显色技术 ,观察SP免疫反应阳性纤维在大鼠脾脏的分布 ;在大鼠背部造成占体表面积 2 0 %的深Ⅱ度烫伤 ,烫伤后不同时间 ,采用放射免疫分析法测定大鼠脾内SP含量的变化 .结果 :在大鼠脾脏内有较丰富的SP免疫反应阳性神经纤维 ,多呈串珠状走行于淋巴组织中 ;与对照组比较 ,大鼠烫伤后脾脏SP含量 [(ng·g-1) 3 0min ,13 .7± 4 .3vs 2 0 .1± 3 .2 ,P <0 .0 5 ;60min ,11.8± 4 .4vs 18.2± 7.5 ,P <0 .0 5 ;12 0min ,7.6± 2 .2vs17.1± 7.5 ,P <0 .0 1;2 4 0min,8.1± 2 .6vs16.5± 6.4 ,P <0 .0 1]明显下降 .结论 :大鼠脾脏内含有较丰富的SP免疫反应阳性神经纤维 ,烫伤后脾内SP含量明显下降 ,提示SP可能是导致烧伤后机体免疫功能抑制的因素之一 .

AIM: To investigate the distribution and content change of substance P (SP) immunoreactive nerve fibers in the rat spleen post burn and to explore the relation between SP and immunological functions. METHODS: The distribution of SP immunoreactive nerve fibers was observed by the immunohistochemical ABC method combined with glucose oxidase DAB nikel enhancement technique. The rats were inflicted on the back with 20% TBSA of Ⅱ degree scalding by boiling water. The post burn changes in the tissue content of the SP in the spleen were determined by radioimmunoassay (RIA). RESULTS: The rat spleen was richly innervated by positive SP immunoreactive nerve fibers. The tissue content of SP in the spleen decreased significantly ( P <0.01) at 30, 60, 120 and 240 minutes post burn. CONCLUSION: The rat spleen is richly innervated by positive SP immunoreactive nerve fibers and the content of SP in the spleen decreased significantly post burn. SP may play the role of enhancing immunological function.