腺病毒介导HSV-tk/GCV系统杀伤脑胶质瘤C6细胞

Killing effect of adenovirus mediated HSV tk/GCV system on glioma C6 cell

目的 :观察腺病毒介导的单纯疱疹病毒胸腺嘧啶核苷激酶 /丙氧鸟甘 (herpessimplexvirusthymidinekinase/ganci clovir HSV tk/GCV)系统对脑胶质瘤细胞的杀伤作用 .方法 :将AdCMVLacZ感染胶质瘤细胞C6,X gal染色 ,测定腺病毒的转染率 .AdCMVtk感染C6细胞后加入 10mg·L-1GCV ,观察肿瘤细胞存活率及旁观者效应 .结果 :tk基因对C6细胞的转染率随着腺病毒的感染复数 (MOI)量增加而增加 (r=0 .819,P<0 .0 5 ) ,10 0 %转染所需要的MOI为 10 0 .AdCMVtk/GCV对C6细胞的杀伤强度与AdCMVtk的量成正相关 (r =0 .870 ,P <0 .0 5 ) .此杀伤作用存在旁观者效应 ,且较强 .结论 :腺病毒介导的HSV

AIM: To observe killing action of HSV tk/GCV system on glioma cells. METHODS: C6 cells were transfected by adenovirus with LacZ gene, then stained with X gal for measuring the adenovirus transfected efficiency. C6 cells survival and bystander effect were observed after adding 10 mg·L -1 GCV to transfected cells. RESULTS: The more the multiplicity of infection (MOI), the more the adenovirus transfected efficiency to C6 cells ( r=0.819, P <0.05). 100% transfected efficiency needed MOI 100. The killing action of HSV tk/GCV on C6 cells, depended on the amount of AdCMVtk ( r=0.870, P <0.05). There was high bystander effect. CONCLUSION: Adenovirus mediated HSV tk/GCV system has good killing effect on glioma C6 cells.