葡多酚对N-亚硝基化合物诱变性的抑制作用

Inhibitory Effect of Grape Procyanidin on the Mutagenicity of N-nitroso Compounds

背景与目的:探讨葡多酚(grape procyanidin,GPC)对N-亚硝基化合物诱变性的抑制作用。材料与方法:采用0.5％的亚硝酸钠经口灌胃诱发大鼠肝细胞突变,2个GPE实验组同时经口分别给予GPC 100和10 mg/kg,8周后杀鼠,用免疫组织化学技术检测肝细胞突变型P53蛋白表达水平和用微核分析技术检测骨髓嗜多染红细胞微核率。结果:诱变损伤组突变型P53蛋白表达的阳性细胞平均值为84.95±6.33(个),骨髓嗜多染红细胞微核率为37.40‰,高剂量GPE组分别为16.30±1.63(个)和8.40‰,低剂量GPC组则分别为57.50±4.68(个)和24.20‰,高、低剂量GPC组与诱变损伤组之间的差异均有显著性(P<0.01)。结论:GPC可降低亚硝酸钠引发的突变型P53蛋白表达和骨髓嗜多染红细胞微核率水平,GPC对N-亚硝基化合物的诱变性有抑制作用。

BACKGROUND & AIM:To study the inhibitory effect of grape procyanidin(GPC) on the mutagenicity of N - nitroso compounds. MATERIAL AND METHODS: Wistar rats were given 0.5 % NaNO2 to induce hepatocytes' mutation, and GPC was given by 100 and 10 mg/ kg respectively through mouths into the rats of the two experimental groups at the same time. After eight weeks, rats were killed and immunohistochemistry method was applied to measure the expression level of mutant P53 protein in rats' hepatic cells , and bone marrow micronucleus method was applied to measure the rate of bone marrow micronucleus.RESULTS: The number of positive cells of mutant P53 protein expressed in the induced - injury group was 84.95±6.33, and the rate of bone marrow micronucleus was 37.40 ‰, and those of the high - dose GPC group were 16.30±1.63 and 8.40 ‰ and the low - dose GPC group were 57.50±4.68 and 24.20 ‰. The ifferences between the two GPC groups and the induced - injury group had statistical significance(P < 0.01) . CONCLUSION: GPC can decrease the expression level of mutant P53 protein and the rate of bone marrow micronucleus induced by NaNO2 ,and it has an inhibitory effect on mutagenicity of N - nitroso compounds.