藏妇康栓的质量标准研究

Study on quality standard for Zangfukang Suppository

目的 :制定藏妇康栓 (马尿泡 ,花椒 ,棘豆 ,等 )的质量标准。方法 :采用薄层色谱法对处方中马尿泡、花椒、棘豆等进行定性鉴别 ;采用高效液相色谱法对方中马尿泡中的莨菪碱进行含量测定。结果 :以羧甲基纤维素钠为黏合剂的硅胶G薄层板 ,以醋酸乙酯 甲醇 浓氨水 (17∶2∶1)为展开剂 ,可进行马尿泡的鉴别研究 ,以环己烷 醋酸乙酯 甲酸 (3 0∶15∶1)为展开剂 ,可进行花椒的鉴别研究 ,以环己烷 醋酸乙酯 甲醇 (50∶10∶1)为展开剂 ,可进行棘豆的鉴别研究 ;莨菪碱在 2 .5～2 5μg范围内线性关系良好 ,r =0 .9999,平均回收率 99.59% ,RSD =1.3 4 %。 结论 :该方法准确可靠地进行定性、定量检测 。

AIM: To establish the method for quality control of Zangfukang Supposit ory (Radix Przewalskii tanguticae, Pericarpium Zanthoxyli, Herba Oxytropis falca tae, etc.). METHODS: Radix Przewalskii tanguticae, Pericarpium Zanthoxyli, Her ba Oxytropis falcatae were identified by TLC, and the content of hyoscyamine in Radix Przewalskii tanguticae was determined by HPLC. RESULTS: The ethyl acetate-methanol-ammonia(17∶2∶1) was applied as the developer for the identification of Radix Przewalskii tanguticae on silica gel G coating plate. The cyclohexane-ethyl acetate-formic acid (30∶15∶1) was applied as the developer for the identification of Pericarpium Zanthoxyli on s ilic a gel G coating plate. The cyclohexane-ethyl acetate-formic acid (50∶10∶1) w as taken as the developer for the identification of Herba Oxytropis falcatae als o on silica gel G coating plate. Hyoscyamine showed a good linear relationship a t a range of 2.5～25μg,r=0.9999. The average recovery was 99.59%, and RSD was 1.34%. CONCLUSION: The methods are accurate and reliable, and can control the quality of this preparation effectively.