共转化法剔除转基因小麦中的bar基因

Excision of bar Gene from Transgenic Wheat Obtained by Biolistic Co-transformation

利用PCR检测了 2 6 8株转小麦黄花叶病病毒复制酶基因T3代植株 ,初步筛选出只含有功能基因 (WYMV Nib8基因 )而不含有筛选标记基因 (bar基因 )的转基因小麦植株 2 8棵 ,为转基因小麦的安全性种植提供了保障。同时对无标记基因的转基因植株进行了叶片涂抹除草剂验证 。

Transgenic crops have developed in commercialized scale in some countries, but their biosafety have attracted public concerns in the world at the same time. Therefore, it is becoming more and more important to eliminate the selective genes because of their negative functions, such as antibiotics resistance and herbicide resistance. 268 T 3 transgenic wheat plants of Yangmai158 containing wheat yellow mosaic virus resistance gene and bar selection gene, which were obtained by biolistic particle co transformation, were analyzed by PCR in this study, and 28 plants only with the target gene were identified finally. Moreover, leaf painting of herbicide was applied to confirm the marker free transgenic plants. It could be more efficient to combine leaf painting and PCR to select bar free transgenic wheat.