高效液相色谱法测定中药滴水珠中麻黄碱的含量

Determination of Ephedrine in Pinellia cordata N.E. Brown by HPLC

目的 测定中药滴水珠中麻黄碱的含量。方法 采用反相高效液相色谱法 ,以 Phenom enex ODS柱为分析柱 ,甲醇 -水 (加磷酸调 p H至 3.5 ) (6 5∶ 35 )为流动相 ,流速 :0 .6 m l· min- 1 ;检测波长为 2 13nm ,柱温 30℃。结果 在 2 0～ 10 0μg· ml- 1 范围内 ,色谱峰面积与对照品浓度呈良好的线性关系 ,r=0 .999;平均回收率为 :91.2 0 % ,RSD为 :1.2 (n=5 )。结论 该方法简易准确 ,重现性好 ,在本色谱条件及样品处理方法下 。

Objective To establish a HPLC method for the determination of ephedrine in Pinellia cordata N.E. Brown.Method The HPLC was conducted on a Phenomenex ODS C 18 column ( 5 μm, 250 mm× 4.6 mm ) with methanol-water solution ( 65∶35 ) (adjusted to pH 3.5 with phosphoric acid ) as mobile phase, flow rate 0.6 ml·min -1,The detective wavelength was 213 nm and column temperature at 30 ℃.Result The peak areas showed a good correlation with concentration at the range of 20～100 μg·ml -1,r=0.999;The average recovery was 91.20%,RSD=1.2%(n=5).Conclusion The method was simple, accurate and reproducible.Under this experimental condition,the ephedrine in Pinellia cordata N.E.Brown was separated very well and determined accurately.