产肠毒素性大肠杆菌肠毒素的研究概况

Research Survey of Enterotoxin in Enterotoxigenic Escherichia coli

本文分析了大肠杆菌耐热肠毒素 (heat-stable,ST)和不耐热肠毒素 (heat- labile,LT)生物学特性、分子水平机制及其应用价值。一方面着重讲述 LT作为粘膜免疫佐剂的研究前景 ,利用体外定点突变方法构建不同的突变株并且均具有不同程度的佐剂活性 ,是霍乱毒素 (Choleratoxin,CT)很有希望的替代品。另一方面讲述ETEC基因工程亚单位疫苗的研究概况。ETEC只有 LT、ST两类肠毒素 ,通过不同方式构建融合基因探讨对 ETEC性腹泻防治效果。目前 ,这两方面均卓有成效 。

The text analysed biological characteristics,molecular mechanisms and application of heat labile(LT)and heat stable(ST)enterotoxin.First of all,different mutants of LT that have been demonstrated as mucosal adjuvants were constructed by site directed mutagenesis and proved to be the excellent candidates of CT.Next,genetically engineered vaccine of fused LT and ST,which was beneficial to preventing and treating diarrhea, was constructed .These results give a base for further research on enterotoxin in ETEC.