苦马豆素对人食道癌Eca-109细胞体外生长的抑制试验

Inhibition Effect of Swainsonine on Human Esophagus Carcinoma Cell Line Eca-109 in vitro

探讨苦马豆素 (SW)对人食道癌 Eca-10 9细胞生长的抑制作用 ,以揭示 SW治疗食道癌的作用机制。选用不同剂量的 SW与食道癌Eca- 10 9细胞共同培养不同时间后 ,用 MTT法观察 SW对食道癌 Eca- 10 9细胞生长的抑制作用 ;细胞 DNA经特异性荧光染色后 ,用流式细胞仪分析细胞周期变化。结果 :SW对食道癌Eca- 10 9细胞生长的半数抑制浓度 IC50 <2 .5μg/ m L;细胞周期分析 ,Eca- 10 9细胞 G1期细胞增多 ,S期细胞减少。结果表明 :SW可通过抑制人食道癌 Eca- 10 9细胞系生长达到抑制肿瘤的目的。

We investigated the inhibition effect of swainsonine(SW) on human esophagas carcinoma cell line to explorie mechanism of treatment of esophagus carcinoma.Methods A human esophagus carcinoma cell line Eca 109 was cultured in full medium with variable doses of SW for different hours. The inhibition effect of SW on esophagus carcinoma cell line Eca 109 was examined with MTT. Cell cycle analysis was performed on flow cytometry (FCM).Results The IC 50 of SW on esophagus carcinoma cell Eca 109 was lower than 2.5 μg/mL.The analysis of cell cycle indicated that the number of the cells was increased in the G1 phase, but decreased in the S phase of the cell cycle.Conclusion SW may inhibit the growth of human esophagus carcinoma cell line Eca 109,which led to the death of the cancer cell.