制备性配位交换树脂的合成

Synthesis of Ligand-Exchange Chromatography Resins

为满足中性氨基酸制备性层析的要求,本研究合成出三种具有不同配位基团的含铜配位交换层析树脂,其担体材料分别为Sephadex G-25,多孔玻璃和聚苯乙烯磺酸树脂。由于在合成过程中采用了新的合成路线和添加适量的有机溶剂,促进了有机反应物在水中的溶解度,提高单位柱载量达33%,并且有良好的稳定性和机械强度,满足了制备性层析要求。

In order to suffice the needs of the preparative ligand-exchange chromatography of neutral amino acids, three types of ligand-exchange resins loading copper ion with different function groups were synthesized by using Sephadex G-25, porous glass and polystyrenesutfonic acid resin respectively. Because of the adaption of the new synthesis technologies, and the addition of adequate amound of organic aolvent, the condition of reactions was improved and the solubility of organic reagents was increased in the aqueos solution, so that the unit column load was increased by 33%, The resins took more better stability and mechanical strength, and so met the demands of preparative chromatography.