摘要
目的 :探讨二仙汤不同时间对肾阳虚大鼠垂体促肾上腺皮质激素 (ACTH)基因表达的影响。方法 :造模大鼠随机分成模型组、二仙汤组 ,在喂药后不同时间 (1 0d ,2 0d ,30d) ,用逆转录聚合酶链反应 (RT PCR)方法 ,检测二仙汤对垂体组织ACTH基因表达的影响。结果 :二仙汤可以上调垂体组织ACTH基因表达 (P <0 0 5 )。结论 :二仙汤通过上调ACTHmRNA表达治疗肾阳虚证是其作用机理之一。
Objective: To study the influence of Er xian Decoction on gene expression of ACTH in pituitary tissue in kidney yang deficiency rats at different time Methods: Kidney yang deficiency rats were randomly divided into model group and Er Xian Decoction group Using reverse transcriptase polymerase chain reaction (RT PCR) to observe the effect of Er Xian decoction on the ACTH mRNA in pituitary tissue that having been treated at different time (10d, 20d, 30d). Results: Er xian Decoction can treat the kidney-yang Deficiency through up regulating the expression of ACTH mRNA and is one of the therapeutic mechanisms
出处
《中国医药学报》
CSCD
2003年第12期716-718,共3页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
浙江省自然科学基金资助项目