摘要
利用^(15)N-同位素技术研究转PEPC基因水稻及无PEPC基因水稻在接种不同固氮菌后的光合特性、产量性状和固氮能力的变化。结果表明:接种水稻联合固氮工程菌处理与未接种固氮菌处理相比,转PEPC基因水稻的PEPC活性、PS Ⅱ光化学效率(Fv/Fm)、净光合速率均明显提高,生物学产量和经济产量也相应增加了38.08%和26.86%;与无PEPC基因水稻相比,转PEPC基因水稻能明显提高水稻联合固氮工程菌的固氮效率。这种水稻光合与固氮相互促进的机理值得深入研究。
The photosynthetic properties, yield character of PEPC transgenic rice inoculated nitrogen-fixing bacteria and nitrogen-fixing capacity of nitrogen-fixing bacteria inoculated in PEPC transgenic rice were studied by using l5 N-isotope tracer technique. The results showed that the PEPC activity, PS Ⅱ photochemical efficiency and net photosynthetic rate of PEPC transgenic rice inoculated nitrogen-fixing bacteria increased significantly under high light intensity . The dry matter of plant and grain yield of PEPC transgenic rice increased 38.08% and 26.86% under it was inoculated with nitrogen-fixing bacteria. Compared with no PEPC gene rice, the PEPC transgenic rice could enhance nitrogen-fixing capacity of nitrogen-fixing bacteria. The mechanism of acceleration between photosynthesis of PEPC transgenic rice and nitrogen fixation of nitrogen-fixing bacteria in PEPC transgenic rice need to be studied deep.
出处
《江苏农业学报》
CSCD
2003年第2期65-69,共5页
Jiangsu Journal of Agricultural Sciences
基金
国家973项目"杂交稻高光效生理育种途径"(J1998010100)