检测六种抗肿瘤抗生素对五种耐药细菌的杀灭效果

Determination of bactericidal effect of six antitumor antibiotics on five antibiotic-resistant bacteria

目的:研究抗肿瘤抗生素对耐药细菌的杀灭效果。方法:选用6种抗肿瘤抗生素平阳霉素、放线菌素D、丝裂霉素、柔红霉素、多柔比星和表柔比星,用微量方法检测其对临床常见的5种耐药细菌金黄色葡萄球菌、绿脓假单胞菌、粪肠球菌、阴沟肠杆菌和肺炎克雷伯菌的最低抑菌浓度(MIC)和最低杀菌浓度(MBC)。结果:6种抗肿瘤抗生素对耐药性金黄色葡萄球菌有较强的杀灭作用;对其他4种耐药细菌有不同程度的杀灭作用,MIC为:4.9×10-3～2.9×10-6mol/L;MBC为:7.5×10-4～2.9×10-6mol/L。结论:6种抗肿瘤抗生素抗耐药细菌的作用依次为:丝裂霉素>柔红霉素>平阳霉素>多柔比星>放线菌素D>表柔比星。

Objective: To study bactericidal effect of antitumor antibiotics on the antibiotic-resistant bacteria. Methods: Minimal inhibitory concentration (MIC ) and minimal bactericidal concentration ( MBC ) of six antitumor antibiotics (mitomycin, daunorubicin, pingyangmycin, doxorubicin, dactinomycin and epirubicin) against five antibiotic-resistant bacteria (Staphylococcus aureus, Pseudomonas aeruginosa, Strep tococcus faecalis , Enterobacter cloacae, Klebsiella pneumoniae) were determined by micromethod. Results: Six antitumor antibiotics had bactericidal effect on antibiotic-resistant bacteria. Their MIC values were 4. 9×10-3-2. 9×10-6mol/L; MBC values were 7. 5×10-4-2. 9×10-6mol/L. Conclusion: The 6 antitumor antibiotics had strong bactericidal effect on the resistant Staphylococcus aureus. The bactericidal effects in decreasing order are: mitomycin>daunorubicin>pingyangmycin>adriamycin>dactinomycin>epirubicin.