慢性捆绑应激对大鼠学习记忆能力及海马TNF-α表达的影响

Effects of Chronic Restraint Stress on the Learning and Memory Ability and on the Expression of TNF-α in Hippocampus

目的探讨学习记忆能力与海马细胞因子TNF -α的关系。方法把大鼠分为应激组和正常组 ,应激组大鼠每天捆绑 6h ,持续 2 1d ,第 2 2d用Y迷宫测试两组大鼠学习记忆能力 ,用原位杂交细胞化学显示两组大鼠海马TNF -αmRNA阳性细胞。结果应激组大鼠学习记忆成绩明显下降 (P <0 .0 5 ) ,其海马TNF -αmRNA阳性细胞数明显高于正常组。结论应激组大鼠学习记忆下降与其海马相关细胞因子TNF

Objective To investigate the relationship between TNF α and learning and memory ability.Methods Rats were divided into stress and control groups.The stress rats were restrained for 6 hours per day for 21 days.In both groups,the behavior of rats were tested by Y maze,and the expression of the TNF α mRNA in hippocampus were observed by in situ hybridization techniques.Results The learning and memory ability of the stress rats were impaired (P<0.05).The expression of TNF α mRNA in stress hippocampus was stronger than that of control rats.Conclusion The impairment of learning and memory ability of the stress rats may be related to the change of the TNF α mRNA in hippocampus of the rats.