褪黑素对氧化诱导人淋巴细胞凋亡相关基因表达的影响

The Effect of Melatonin on Human Lymphocytes Apoptosis Induced by H_2O_2

目的研究抗氧化剂褪黑素 (MT)对H2 O2 诱导人淋巴细胞凋亡的作用。方法用免疫细胞化学的方法检测凋亡相关基因bcl-2、bax的蛋白表达。结果H2 O2 处理细胞后bcl-2基因表达轻度增高 ,加入MT后明显增高 ,而bax基因表达明显增加 ,MT干预后明显下降。结论H2 O2 可诱导人淋巴细胞凋亡 ,MT可使H2 O2 诱导的细胞bcl-2基因的蛋白表达明显增高 ,并明显降低bax基因的蛋白表达 ,从而抑制淋巴细胞凋亡。

Objective To study the effects of melatonin(MT) on human lymphocytes apoptosis induced by H 2O 2.Methods The protein expression of bcl-2 and bax genes were detected by immunocytochemistry.Results The protein expression of bcl-2 gene increased slightly after H 2O 2 treatment,but it increased significantly after MT treatment.The protein expression of bax gene increased significantly after H 2O 2 treatment,but it decreased significantly after MT treatment.Conclusion Oxidative stress can induce apoptosis in lymphocytes, and this induction can be partly prevented by melatonin.